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Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » PI3 Kinase p85 alpha antibody, pAb, Rabbit

PI3 Kinase p85 alpha antibody, pAb, Rabbit

PI3 Kinase P85 Alpha Antibody, PAb, Rabbit

Western blot analysis of cell lysate using PI3 Kinase p85 alpha Antibody (GenScript, A01524, 2 µg/ml)

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Predicted Size: 85 KD
Observed Size: 85 KD

PI3 Kinase P85 Alpha Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using PI3 Kinase p85 alpha antibody, pAb, Rabbit (GenScript, A01524; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms p85 alpha antibody;
Description Phosphoinositide 3-kinase (PI3K) catalyzes the phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit (p85). The catalytic subunits of PI3K include p110 alpha, p110 beta, p110 gamma, and p110 delta, while the regulatory subunits contain p85 alpha and p85 beta. p85 alpha regulates the catalytic activity of p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain. The p85 alpha has been implicated in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.GenScript PI3 Kinase p85 alpha Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 600–650 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 600-650 of human PI3 Kinase p85 alpha (Swiss Prot: P27986).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript PI3 Kinase p85 alpha Antibody detects endogenous levels of human PI3 Kinase p85 alpha. Predicted to react with mouse and rat PI3 Kinase p85 alpha according to sequence homology.
Positive Control: Ramos
Species Reactivity Human
Predicted Band Size 85 KD
Observed Band Size 85 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.


PI3 Kinase P85 Alpha Antibody, PAb, Rabbit
PI3 Kinase P85 Alpha Antibody, PAb, Rabbit

Western blot analysis of cell lysate using PI3 Kinase p85 alpha Antibody (GenScript, A01524, 2 µg/ml)

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Predicted Size: 85 KD
Observed Size: 85 KD

PI3 Kinase P85 Alpha Antibody, PAb, Rabbit
PI3 Kinase P85 Alpha Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using PI3 Kinase p85 alpha antibody, pAb, Rabbit (GenScript, A01524; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01524
Size
Price
40 ug $99.00
100 ug $199.00
 
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