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Home » Gene Synthesis & Molecular Biology Services » CTCF antibody, pAb, Rabbit

CTCF antibody, pAb, Rabbit

Synonyms 11 zinc finger protein antibody;
Description CTCF (CCCTC-binding factor) is a highly conserved zinc finger protein which plays an important role in transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation depending on the DNA context of the binding site. It contains a DNA-binding domain composed of 11 zinc-fingers and the DNA target sequences recognized by CTCF which are fairly long and strikingly diverse. CTCF can be phosphorylated by the protein kinase CK2, as well as poly(ADP-ribosyl)ated. The latter modification regulates its activity as a chromatin insulator. GenScript CTCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150–200 of human CTCF (Swiss Prot: P49711).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 150-200 of human CTCF (Swiss Prot: P49711).
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript CTCF Antibody detects endogenous levels of human CTCF. Predicted to react with rat CTCF according to sequence homology.
Positive Control: Ramos, K562 and A431
Species Reactivity Human
Predicted Band Size 83 KD
Observed Band Size 85 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml.
Other Applications: user optimized.

  • CTCF Antibody, PAb, Rabbit
  • CTCF Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using CTCF Antibody (GenScript, A01529, 2 µg/ml)

    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

    Predicted Size: 83 KD
    Observed Size: 85 KD




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Cat. No. A01529
Size
Price
40 ug $99.00