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Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » THETM alpha Tubulin Antibody [Biotin], mAb, Mouse

THE™ alpha Tubulin Antibody [Biotin], mAb, Mouse

*This product has been discontinued!*
Synonyms THE™ α-Tubulin Antibody [Biotin], mAb, Mouse;
Description GenScript THETM α-tubulin Antibody[Biotin], mAb, Mouse is the biotin conjugated THETM α-tubulin Antibody, mAb, Mouse(A01410) purified from mice ascites by protein A affinity column.
Host Species Mouse
Conjugation Biotin
Immunogen KLH-coupled synthetic peptide within residues 400-450 of human α-tubulin (Swiss Prot: P68363).
Fusion Partner Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells

Subclass Mouse IgG2b, k
Clone ID 1F4E3
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript THETM α-tubulin Antibody[Biotin], mAb, Mouse recognizes Mouse, Human, Hamster, Monkey, Rabbit and Pig. The antibody has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-0.5 µg/ml
Other applications: user-optimized

Cat. No. Product Name Quantity Price
THE™ alpha Tubulin Antibody, mAb, Mouse - 100 ug
THE™ alpha Tubulin Antibody, mAb, Mouse - 10 ug
Martinez-Espinosa PL, et al. Knockout of the BK β2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. J Gen Physiol. 2014Qct.;144(4):275-95

J Capecci., et al. Function of V-ATPase a Subunit Isoforms in Invasiveness of MCF10a and MCF10CA1a Human Breast Cancer Cells. J Biol Chem. 2013 Nov;288(45):32731-41.

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