|Synonyms||H1N1 Polymerase PB2 of Influenza A Virus antibody;|
|Description||Influenza A (H1N1) virus, a subtype of influenzavirus A, is the most common cause of influenza (flu) in humans. A strain of swine-origin H1N1 was responsible for the 2009 flu pandemic. H1N1PB2 protein is a well-documented component of the viral polymerase complex required for virus replication. The PB2 protein is involved in host range restriction and pathogenicity.
GenScript H1N1 PB2 Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of Influenza A (H1N1) 2009 virus PB2 protein (GenBank: CY040006).
|Antigen Species||Influenza A|
|Immunogen||KLH-coupled synthetic peptide from N-terminal of Influenza A (H1N1) 2009 virus PB2 protein (GenBank: CY040006).|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript H1N1 PB2 Antibody detects influenza A (H1N1) PB2 protein.|
|Species Reactivity||Influenza A virus (H1N1)|
|Predicted Band Size||100 KD|
|Observed Band Size||100 KD|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml.
Other Applications: user-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)