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Home » Gene Synthesis & Molecular Biology Services » EndoG antibody, pAb, Rabbit

EndoG antibody, pAb, Rabbit

EndoG Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using EndoG Antibody (GenScript, A01588, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 33 KD
Observed Size: 24 KD

EndoG Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using EndoG Antibody (GenScript, A01588; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms Endonuclease G Antibody;
Description Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis.
GenScript Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.
Positive Control: Ramos, mouse kidney
Species Reactivity Human and mouse
Predicted Band Size 33 KD
Observed Band Size 24 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user-optimized

  • EndoG Antibody, PAb, Rabbit
  • EndoG Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using EndoG Antibody (GenScript, A01588, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 33 KD
    Observed Size: 24 KD

  • EndoG Antibody, PAb, Rabbit
  • EndoG Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using EndoG Antibody (GenScript, A01588; shaded histogram)
    or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01588
Size
Price
40 ug $99.00