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EndoG antibody, pAb, Rabbit

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Western blot analysis of tissue lysates using EndoG Antibody (GenScript, A01588, 1 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 33 KD
Observed Size: 24 KD

Flow cytometric analysis of Ramos cells using EndoG Antibody (GenScript, A01588; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

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Overview

Synonyms	Endonuclease G Antibody;
Description	Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis. GenScript Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249).
Host Species	Rabbit
Antigen Species	Human
Conjugation	Unconjugated
Immunogen	KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249)
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology. Positive Control: Ramos, mouse kidney
Species Reactivity	Human and mouse
Predicted Band Size	33 KD
Observed Band Size	24 KD
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized