Western blot analysis of tissue lysates using EndoG Antibody (GenScript, A01588, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 33 KD
Observed Size: 24 KD
Flow cytometric analysis of Ramos cells using EndoG Antibody (GenScript, A01588; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.
Synonyms | Endonuclease G Antibody; |
Description | Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis. GenScript Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249). |
Host Species | Rabbit |
Antigen Species | Human |
Conjugation | Unconjugated |
Immunogen | KLH-coupled synthetic peptide within residues 200–250 of human EndoG (Swiss Prot: Q14249) |
Purification | Immunoaffinity chromatography |
Subclass | Rabbit IgG |
Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Specificity | EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology. Positive Control: Ramos, mouse kidney |
Species Reactivity | Human and mouse |
Predicted Band Size | 33 KD |
Observed Band Size | 24 KD |
Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western blot: 0.5-1 µg/ml Flow cytometry: 1-3 µg for 1 x 106 cells Other applications: user-optimized |
Western blot analysis of tissue lysates using EndoG Antibody (GenScript, A01588, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 33 KD
Observed Size: 24 KD
Flow cytometric analysis of Ramos cells using EndoG Antibody (GenScript, A01588; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.
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