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Catalog Products » Antibodies » Primary Antibodies » Cell Marker Antibodies » SCF antibody, pAb, Rabbit

SCF antibody, pAb, Rabbit

SCF Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using SCF Antibody (GenScript, A01596, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 33 KD
Observed Size: 33 KD

SCF Antibody, PAb, Rabbit

Flow cytometric analysis of Ramos cells using SCF Antibody (GenScript, A01596; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

Synonyms SCF Antibody;
Description Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis, and fertility. It exerts its activity at the early stages of hematopoiesis. Specifically, SCF stimulates the proliferation of mast cell and mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. In addition, SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways.
GenScript SCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.
Positive Control: Ramos cell, mouse kidney tissue lysate
Species Reactivity Human and mouse
Predicted Band Size 33 KD
Observed Band Size 33 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Other applications: user optimized

  • SCF Antibody, PAb, Rabbit
  • SCF Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using SCF Antibody (GenScript, A01596, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 33 KD
    Observed Size: 33 KD

  • SCF Antibody, PAb, Rabbit
  • SCF Antibody, PAb, Rabbit

    Flow cytometric analysis of Ramos cells using SCF Antibody (GenScript, A01596; shaded histogram)
    or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.




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Cat. No. A01596
Size
Price
40 ug $99.00