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Home » Gene Synthesis & Molecular Biology Services » UBE3A antibody, pAb, Rabbit

UBE3A antibody, pAb, Rabbit

UBE3A Antibody, PAb, Rabbit

Western blot analysis of tissue lysates using UBE3A Antibody (GenScript, A01603, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 101 KD
Observed Size: 75 KD

UBE3A Antibody, PAb, Rabbit

Western blot analysis of cell lysates using UBE3A Antibody (GenScript, A01603, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 101 KD
Observed Size: 75 KD

UBE3A Antibody, PAb, Rabbit

Flow cytometric analysis of HEK293 cells using UBE3A Antibody (GenScript, A01603; shaded histogram)
or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

UBE3A Antibody, PAb, Rabbit

Immunohistochemistry analysis of human placenta tissue slide (Paraffin embedded) using UBE3A antibody, pAb, Rabbit (GenScript, Cat.No. A01603, 10 µg/ml)

UBE3A Antibody, PAb, Rabbit

Immunohistochemistry analysis of human tonsil tissue slide (Paraffin embedded) using UBE3A antibody, pAb, Rabbit (GenScript, Cat.No. A01603, 10 µg/ml)

Synonyms Ubiquitin protein ligase E3A Antibody;
Description UBE3A (Ubiquitin protein ligase E3A) is one member of the E3 ubiquitin-protein ligase family which plays a role in defining the substrate specificity of the ubiquitin-proteasome degradation system. UBE3A can interact directly with the growth and tumor-suppressive protein, p53 or indirectly as part of a human papillomavirus type 16 or 18 E6 protein complex. Mutations within the UBE3A gene are responsible for some cases of Angelman syndrome. Alternative splicing of UBE3A gene results in three transcript variants encoding three isoforms with different N-termini.
GenScript UBE3A antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human UBE3A (Swiss Prot: Q05086).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from N-terminal of human UBE3A (Swiss Prot: Q05086)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity UBE3A Antibody detects endogenous levels of human and mouse UBE3A.
Positive Control: Hela, A549, HepG2, HT29 and mouse brain tissue
Species Reactivity Human and Mouse
Predicted Band Size 101 KD
Observed Band Size 75 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Flow cytometry: 1-3 µg for 1 x 106 cells
Immunohistochemistry: 5-10 µg/ml
Other Applications: user optimized

  • UBE3A Antibody, PAb, Rabbit
  • UBE3A Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using UBE3A Antibody (GenScript, A01603, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 101 KD
    Observed Size: 75 KD

  • UBE3A Antibody, PAb, Rabbit
  • UBE3A Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using UBE3A Antibody (GenScript, A01603, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 101 KD
    Observed Size: 75 KD

  • UBE3A Antibody, PAb, Rabbit
  • UBE3A Antibody, PAb, Rabbit

    Flow cytometric analysis of HEK293 cells using UBE3A Antibody (GenScript, A01603; shaded histogram)
    or with an isotype control antibody (GenScript, A01008; open histogram), followed by R-PE conjugated anti-rabbit IgG.

  • UBE3A Antibody, PAb, Rabbit
  • UBE3A Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human placenta tissue slide (Paraffin embedded) using UBE3A antibody, pAb, Rabbit (GenScript, Cat.No. A01603, 10 µg/ml)

  • UBE3A Antibody, PAb, Rabbit
  • UBE3A Antibody, PAb, Rabbit

    Immunohistochemistry analysis of human tonsil tissue slide (Paraffin embedded) using UBE3A antibody, pAb, Rabbit (GenScript, Cat.No. A01603, 10 µg/ml)




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Cat. No. A01603
Size
Price
40 ug $99.00