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Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » His Tagged Protein Detection & Purification » THETM His Tag Antibody [FITC], mAb, Mouse

THETM His Tag Antibody [FITC], mAb, Mouse

Synonyms THETM His Tag Antibody [FITC], mAb, Mouse;
Description GenScript THETM His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THETM His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species Mouse
Conjugation Fluorescein (FITC)
Immunogen A synthetic peptide HHHHHH coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.
Specificity GenScript THETM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/ml
Flow cytometry: 0.01-0.1 µg/ml
Other applications: User-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  • THE<sup>TM</sup> His Tag Antibody [FITC], MAb, Mouse
  • THE<sup>TM</sup> His Tag Antibody [FITC], MAb, Mouse

    Flow cytometric analysis of transfected HEK293 cell
    expressing His-tagged protein using THETM
    Anti-His-tag [FITC] Monoclonal Antibody (GenScript, A01620;
    shaded histogram) or with a negative control antibody (open
    histogram).


Cat. No.
Product Name
Price
A00174-40
His-tag Antibody, pAb, Rabbit - 40 ug
$99.00
A00186-100
THETM His Tag Antibody, mAb, Mouse - 100 ug
$375.00
A00205-40
His-tag antibody [Biotin], pAb, Rabbit - 40 ug
$99.00
A00612
THETM His Tag Antibody [HRP], mAb, Mouse - 100 ug
$410.00
A00613
THETM His Tag Antibody [Biotin], mAb, Mouse - 100 ug
$410.00
L00436
His Tag ELISA Detection Kit - 1 kit
$298.00
L00439-1
Anti-His Affinity Resin - 1 ml
$399.00
L00439-5
Anti-His Affinity Resin - 5 ml
$1599.00
L00440c
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
$179.00
L00440w
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
$179.00
L00440b
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
$179.00
A01800-100
THETM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$450.00
A01801-100
THETM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$450.00
A01802-100
THETM His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$450.00
 
Oliveira P., et al. Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. strain PCC 6803. J Bacteriol. 2011 Aug;193(15):3804-3814.

An C., et al. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-1939.

Li XH., et al. Expression of Trichoderma viride endoglucanase III in the larvae of silkworm, Bombyx mori L. and characteristic analysis of the recombinant protein. Mol Biol Rep. 2011 Aug;38(6):3897-3902.

Jarmander J, et al. Process optimization for increased yield of surface-expressed protein in Escherichia coli. Bioprocess Biosyst Eng. 2014Feb.;

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For more documents, please visit "Technical Support".

Cat. No. A01620
Size
Price
100 ug $410.00
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