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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM His Tag Antibody [FITC], mAb, Mouse

THETM His Tag Antibody [FITC], mAb, Mouse

Synonyms THETM His Tag Antibody [FITC], mAb, Mouse;
Description GenScript THETM His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THETM His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species Mouse
Conjugation Fluorescein (FITC)
Immunogen A synthetic peptide HHHHHH coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.
Specificity GenScript THETM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/ml
Flow cytometry: 0.01-0.1 µg/ml
Other applications: User-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> His Tag Antibody [FITC], MAb, Mouse
THE<sup>TM</sup> His Tag Antibody [FITC], MAb, Mouse

Flow cytometric analysis of transfected HEK293 cell
expressing His-tagged protein using THETM
Anti-His-tag [FITC] Monoclonal Antibody (GenScript, A01620;
shaded histogram) or with a negative control antibody (open
histogram).


Cat. No.
Product Name
Size
Price
Quantity
A00174-40 His-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00186-100 THETM His Tag Antibody, mAb, Mouse
100 ug
$375.00
A00205-40 His-tag antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00612 THETM His Tag Antibody [HRP], mAb, Mouse
100 ug
$410.00
A00613 THETM His Tag Antibody [Biotin], mAb, Mouse
100 ug
$410.00
L00436 His Tag ELISA Detection Kit
1 kit
$298.00
L00439-1 Anti-His Affinity Resin
1 ml
$399.00
L00439-5 Anti-His Affinity Resin
5 ml
$1599.00
L00440c His Tag Antibody Plate (clear, 8x12 strips)
5 plates
$179.00
L00440w His Tag Antibody Plate (white, 96 wells, nonremovable)
5 plates
$179.00
L00440b His Tag Antibody Plate (black, 96 wells, nonremovable)
5 plates
$179.00
A01800-100 THETM His Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$450.00
A01801-100 THETM His Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$450.00
A01802-100 THETM His Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$450.00
 
Oliveira P., et al. Novel insights into the regulation of LexA in the cyanobacterium Synechocystis sp. strain PCC 6803. J Bacteriol. 2011 Aug;193(15):3804-3814.

An C., et al. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-1939.

Li XH., et al. Expression of Trichoderma viride endoglucanase III in the larvae of silkworm, Bombyx mori L. and characteristic analysis of the recombinant protein. Mol Biol Rep. 2011 Aug;38(6):3897-3902.

Jarmander J, et al. Process optimization for increased yield of surface-expressed protein in Escherichia coli. Bioprocess Biosyst Eng. 2014Feb.;

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Cat. No. A01620
Size
Price
100 ug $410.00
24 hour
 
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