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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM HA Tag Antibody [FITC], mAb, Mouse

THETM HA Tag Antibody [FITC], mAb, Mouse

Synonyms THETM HA Tag Antibody [FITC], mAb, Mouse;
Description GenScript THETM HA Tag Antibody [FITC], mAb, Mouse is FITC conjugated THETM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of HA fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species Mouse
Conjugation Fluorescein (FITC)
Immunogen A synthetic peptide YPYDVPDYA coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 5E11D8
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.
Specificity GenScript THETM HA Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 106 cells
Other applications: User-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> HA Tag Antibody [FITC], MAb, Mouse
THE<sup>TM</sup> HA Tag Antibody [FITC], MAb, Mouse

Flow cytometric analysis of transfected HEK-293 cell
expressing HA-tagged protein using THETM
Anti-HA-tag [FITC] Monoclonal Antibody (GenScript, A01621;
shaded histogram) or with a negative control antibody (open
histogram).


Cat. No.
Product Name
Size
Price
Quantity
A00168-40 HA-tag Antibody, pAb, Goat
40 ug
$99.00
A00169-100 HA-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
A00169-40 HA-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
A00203-40 HA-tag Antibody [Biotin], pAb, Goat
40 ug
$99.00
A01244-100 THETM HA Tag Antibody, mAb, Mouse
100 ug
$287.00
A01296 THETM HA Tag Antibody [HRP], mAb, Mouse
100 ug
$299.00
A01297 THETM HA Tag Antibody [Biotin], mAb, Mouse
100 ug
$299.00
A01806-100 THETM HA Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$344.00
A01807-100 THETM HA Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$344.00
A01808-100 THETM HA Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$344.00
 


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Cat. No. A01621
Size
Price
100 ug $299.00
 
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