THETM HA Tag Antibody [FITC], mAb, Mouse Antibody

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Overview

Synonyms	THE^TM HA Tag Antibody [FITC], mAb, Mouse;
Description	GenScript THE^TM HA Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE^TM HA Tag Antibody, mAb, Mouse (A01244) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of HA fusion proteins by various immunoassays. Absorption Wavelength: 492 nm Emission Wavelength: 518 nm Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species	Mouse
Conjugation	Fluorescein (FITC)
Immunogen	A synthetic peptide YPYDVPDYA coupled to KLH
Purification	Protein A affinity column

Properties

Subclass	IgG1, k
Clone ID	5E11D8
Concentration	1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.
Specificity	GenScript THE^TM HA Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal HA tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 10⁶ cells
Other applications: User-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> HA Tag Antibody [FITC], MAb, Mouse

Flow cytometric analysis of transfected HEK-293 cell
expressing HA-tagged protein using THE^TM
Anti-HA-tag [FITC] Monoclonal Antibody (GenScript, A01621;
shaded histogram) or with a negative control antibody (open
histogram).

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