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Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » GAPDH Antibody, mAb, Mouse

GAPDH Antibody, mAb, Mouse

GAPDH Antibody, MAb, Mouse

Western blot analysis of cell and tissue lysates using GAPDH Antibody, mAb, Mouse (GenScript, A01622, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 36 KD
Observed Size: 36 KD

GAPDH Antibody, MAb, Mouse

Western blot analysis tissue lysates using GAPDH Antibody, mAb, Mouse (GenScript, A01622, 1 µg/ml)
The signal was developed with IRDyTM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 36 KD
Observed Size: 36 KD

Synonyms GAPDH antibody, mAb, Mouse;
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimers and Huntingtons disease.
GenScript GAPDH Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with human GAPDH protein from erythrocytes.
Host Species Mouse
Conjugation Unconjugated
Immunogen Human GAPDH protein from erythrocytes
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2a, κ
Clone ID 3B1E9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript GAPDH Antibody, mAb, Mouse detects endogenous levels of human, pig, cow, goat, and fish GAPDH.
Positive Control: Hela, HepG2, COS7, and pig muscle.
Species Reactivity Human, pig, cow, goat and fish. This product has been not tested with other species yet.
Predicted Band Size 36 KD
Observed Band Size 36 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other Applications: User optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

GAPDH Antibody, MAb, Mouse
GAPDH Antibody, MAb, Mouse

Western blot analysis of cell and tissue lysates using GAPDH Antibody, mAb, Mouse (GenScript, A01622, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 36 KD
Observed Size: 36 KD

GAPDH Antibody, MAb, Mouse
GAPDH Antibody, MAb, Mouse

Western blot analysis tissue lysates using GAPDH Antibody, mAb, Mouse (GenScript, A01622, 1 µg/ml)
The signal was developed with IRDyTM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 36 KD
Observed Size: 36 KD


Cassel JA., et al. Ubiquilin-2 (UBQLN2) binds with high affinity to the C-terminal region of TDP-43 and modulates TDP-43 levels in H4 cells: Characterization of inhibition by nucleic acids and 4-aminoquinolines. Biochim Biophys Acta. 2013 Mar;1834(6):964 - 971.

Shuying Yang., et al. Mx1‐Cre mediated Rgs12 conditional knockout mice exhibit increased bone mass phenotype. Genesis. 2013 Mar;51(3):201-9.

Cassel JA., et al. Characterization of a series of 4-aminoquinolines that stimulate caspase-7 mediated cleavage of TDP-43 and inhibit its function. Biochimie. 2012 Sep;94(9):1974-81.

Martins RP., et al. Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection. J Proteomics. 2012 Jul;75(14):4457-70.

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Cat. No. A01622
Size
Price
40 ug $99.00
24 hour
 
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