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Home » Gene Synthesis & Molecular Biology Services » AMPKα1 Antibody, pAb, Rabbit

AMPKα1 Antibody, pAb, Rabbit

AMPKα1 Antibody, PAb, Rabbit

Western blot analysis of cell lysates using AMPKα1 Antibody, pAb, Rabbit (GenScript, A01623, 1 µg/ml)
Predicted Size: 64 KD
Observed Size: 64 KD

AMPKα1 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanLiver tissue slide (Paraffin embedded) using AMPKα1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01623, 10 ug/ml).

AMPKα1 Antibody, PAb, Rabbit

ImmunohistochemistryanalysisofhumanTestis tissue slide (Paraffin embedded) using AMPKα1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01623, 10 ug/ml).

Synonyms AMPK1 antibody, pAb, Rabbit ;
Description AMP-activated protein kinase (AMPK) plays an important role in the regulation of energy homeostasis. It is activated by an elevated AMP/ATP ratio caused by cellular and environmental stress, such as heat shock, hypoxia, and ischemia. AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, α2; β1, β2; γ1, γ2, γ3). Phosphorylation of AMPKα at Thr172 is the major regulatory site which is required for AMPK activation.
GenScript AMPKα1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide within residues 500–550 of human AMPKα1 (Swiss Prot: Q13131).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide within residues 500–550 of human AMPKα1 (Swiss Prot: Q13131)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript AMPKα1 Antibody, pAb, Rabbit detects endogenous levels of human AMPKα1 and does not cross-react with AMPKα2. It is predicted to react with mouse AMPKα1 according to sequence homology.
Positive Control: HEK293 and MCF-7
Species Reactivity Human
Predicted Band Size 64 KD
Observed Band Size 64 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunohistochemistry:5-10 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: User optimized

  • AMPKα1 Antibody, PAb, Rabbit
  • AMPKα1 Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using AMPKα1 Antibody, pAb, Rabbit (GenScript, A01623, 1 µg/ml)
    Predicted Size: 64 KD
    Observed Size: 64 KD

  • AMPKα1 Antibody, PAb, Rabbit
  • AMPKα1 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanLiver tissue slide (Paraffin embedded) using AMPKα1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01623, 10 ug/ml).

  • AMPKα1 Antibody, PAb, Rabbit
  • AMPKα1 Antibody, PAb, Rabbit

    ImmunohistochemistryanalysisofhumanTestis tissue slide (Paraffin embedded) using AMPKα1 Antibody, pAb, Rabbit (GenScript, Cat. No.A01623, 10 ug/ml).


Cat. No.
Product Name
Price
A01624-40
AMPKγ2 Antibody, pAb, Rabbit - 40 ug
$99.00
 


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Cat. No. A01623
Size
Price
40 ug $99.00