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Catalog Products » Antibodies » Epitope Tag Antibodies » Rho-tag Antibody, pAb, Rabbit

Rho-tag Antibody, pAb, Rabbit

Rho-tag Antibody, PAb, Rabbit

Western blot analysis of N-terminal and C-terminal Rho-tagged fusion proteins using Rho-tag Antibody, pAb, Rabbit (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 65 KD
Observed Size: 65 KD

Rho-tag Antibody, PAb, Rabbit

Western blot analysis of tissue lysate using Rho-tag Antibody (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 40 KD
Observed Size: 40 KD, 20KD

Rho-tag Antibody, PAb, Rabbit

Western blot analysis of C-terminal Rho-tagged fusion proteins using Rho-tag Antibody, pAb, Rabbit (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rho tag Antibody ; Rhodopsin Antibody
Description Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems.
The Rho-tag is an epitope tag consisting of a twenty-residue peptide, MNGTEGPNFYVPFSNKTGVV, which is derived from the first 20aa of bovine rhodopsin. Rho-tag can be used to boost expression levels of some eukaryotic membrane protein.
GenScript Rho-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic Rho-tag epitope peptide.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Developed in rabbit using a KLH-coupled synthetic Rho-tag epitope peptide
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript Rho-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing Rho epitope tags. The antibody also recognizes mouse endogenous rhodopsin proteins.
Predicted Band Size 65 kDa
Observed Band Size 65 kDa
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
IP Protocol(pdf)

Rho-tag Antibody, PAb, Rabbit
Rho-tag Antibody, PAb, Rabbit

Western blot analysis of N-terminal and C-terminal Rho-tagged fusion proteins using Rho-tag Antibody, pAb, Rabbit (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 65 KD
Observed Size: 65 KD

Rho-tag Antibody, PAb, Rabbit
Rho-tag Antibody, PAb, Rabbit

Western blot analysis of tissue lysate using Rho-tag Antibody (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 40 KD
Observed Size: 40 KD, 20KD

Rho-tag Antibody, PAb, Rabbit
Rho-tag Antibody, PAb, Rabbit

Western blot analysis of C-terminal Rho-tagged fusion proteins using Rho-tag Antibody, pAb, Rabbit (GenScript, A01628, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A01628
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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