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Catalog Products » Antibodies » Epitope Tag Antibodies » Nano-tag Antibody, pAb, Rabbit

Nano-tag Antibody, pAb, Rabbit

Nano-tag Antibody, PAb, Rabbit

Western blot analysis of Nano9-tagged and Nano15-tagged fusion proteins using Nano-tag Antibody, pAb, Rabbit (GenScript, A01629, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 42 KD, 30KD
Observed Size: 42 KD, 30KD

Nano-tag Antibody, PAb, Rabbit

Western blot analysis of Nano15-tagged fusion proteins using Nano-tag Antibody, pAb, Rabbit (GenScript, A01629, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 30 KD
Observed Size: 30 KD

Synonyms Nano-Tag Antibody; Nano-tag9 Antibody; Nano-tag15 Antibody;
Description Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems. The Nano-tag is a new streptavidin-binding peptide for both the purification and the detection of Nano-tagged proteins. This peptide possesses nanomolar-affinity for streptavidin and therefore is termed Nano-tag. The nano-tags have two types, Nano-tag15 (MDVEAWLGARVPLVET) and Nano-tag9 (MDVEAWLGAR), which bind to streptavidin with dissociation constants of 4 nM and 17 nM, respectively.
GenScript Nano-tag Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic Nano-tag epitope peptide.This antibody specifically reacts with fusion proteins containing Nano epitope tag. The antibody recognizes both Nano-tag9 and Nano-tag15 fusion protein.
Host Species Rabbit
Conjugation Unconjugated
Immunogen KLH-coupled synthetic Nano-tag epitope peptide
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: user optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Nano-tag Antibody, PAb, Rabbit
Nano-tag Antibody, PAb, Rabbit

Western blot analysis of Nano9-tagged and Nano15-tagged fusion proteins using Nano-tag Antibody, pAb, Rabbit (GenScript, A01629, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 42 KD, 30KD
Observed Size: 42 KD, 30KD

Nano-tag Antibody, PAb, Rabbit
Nano-tag Antibody, PAb, Rabbit

Western blot analysis of Nano15-tagged fusion proteins using Nano-tag Antibody, pAb, Rabbit (GenScript, A01629, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 30 KD
Observed Size: 30 KD




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Cat. No. A01629
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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