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Home » Gene Synthesis & Molecular Biology Services » cPLA2 Antibody, pAb, Rabbit

cPLA2 Antibody, pAb, Rabbit

Synonyms Cytosolic phospholipase A2 antibody, pAb, Rabbit;
Description Cytosolic phospholipase A2 (cPLA2) catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in mitogenesis, differentiation, inflammation, and cytotoxicity. Its catalytic activity is stimulated via phosphorylation of cPLA2 at Ser505 and Ser727. cPLA2 is expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts, and lung endothelium.
GenScript cPLA2 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human cPLA2 (Swiss Prot: P47712).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from C-terminal of human cPLA2 (Swiss Prot: P47712)
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript cPLA2 Antibody, pAb, Rabbit detects endogenous levels of mouse cPLA2. It is predicted to react with human and rat cPLA2 according to sequence homology.
Positive Control: Mouse heart
Species Reactivity Mouse
Predicted Band Size 85 KD
Observed Band Size 65 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml
Other Applications: User optimized

  • CPLA2 Antibody, PAb, Rabbit
  • CPLA2 Antibody, PAb, Rabbit

    Western blot analysis of tissue lysates using cPLA2 Antibody, pAb, Rabbit (GenScript, A01631, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 85 KD
    Observed Size: 65 KD




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Cat. No. A01631
Size
Price
40 ug $99.00