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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse

THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse

Synonyms THETM Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse); THETM Anti-Flag [FITC] Monoclonal Antibody (Mouse);
Description GenScript THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THETM DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species Mouse
Conjugation Fluorescein (FITC)
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 106 cells
Other applications: User-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> DYKDDDDK Tag Antibody [FITC], MAb, Mouse
THE<sup>TM</sup> DYKDDDDK Tag Antibody [FITC], MAb, Mouse

Flow cytometric analysis of transfected HEK-293 cell expressing DYKDDDDK-tagged protein using
THETM Anti-DYKDDDDK [FITC] Monoclonal Antibody (GenScript, A01632; shaded histogram) or with a
negative control antibody (open histogram).


Cat. No.
Product Name
Size
Price
Quantity
A00170-40 DYKDDDDK-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00187-100 THETM DYKDDDDK Tag Antibody, mAb, Mouse
100 ug
$139.00
A00187-200 THETM DYKDDDDK Tag Antibody, mAb, Mouse
200 ug
$256.00
A01428-100 THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse
100 ug
$149.00
L00425 Anti-DYKDDDDK IP Resin
1 ml
$199.00
L00432-1 Anti-DYKDDDDK G1 Affinity Resin
1 ml
$281.00
L00432-25 Anti-DYKDDDDK G1 Affinity Resin
25 ml
$2591.00
L00432-5 Anti-DYKDDDDK G1 Affinity Resin
5 ml
$663.00
L00432-10 Anti-DYKDDDDK G1 Affinity Resin
10 ml
$1193.00
L00455C DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip)
5 plates
$229.00
L00455W DYKDDDDK Tag Antibody Plate (White, 96-well)
5 plates
$229.00
L00455B DYKDDDDK Tag Antibody Plate (Black, 96-well)
5 plates
$229.00
A01809-100 THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$167.00
A01810-100 THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$167.00
A01811-100 THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$167.00
 
Pumroy RA., et al. Nucleoporin Nup50 Stabilizes Closed Conformation Of Armadillo Repeat 10 In Importin 5. J Biol Chem. 2012 Jan;287(3):2022-31.

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For more documents, please visit "Technical Support".

Cat. No. A01632
Size
Price
100 ug $149.00
24 hour
 
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