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Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » RNA Polymerase II CTD Repeat Antibody (Phospho-Ser5), pAb, Rabbit

RNA Polymerase II CTD Repeat Antibody (Phospho-Ser5), pAb, Rabbit

Synonyms RNA polymerase II subunit B1 Antibody, pAb, Rabbit;
Description RNA polymerase II is an integral part of the transcription initiation complex. It possesses a unique C-terminal domain (CTD) consisting of repeats of the consensus heptapeptide sequence YSPTSPS. The CTD domain interacts with a large multisubunit complex that contains TATA-binding protein (TBP). Phosphorylation of RNA polymerase IIs largest C-terminal domain (CTD) is a key event during mRNA metabolism.
GenScript RNA polymerase II CTD repeat Antibody (Phospho-Ser5), pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic phosphopeptide corresponding to the residues surrounding serine 5 of human RNA polymerase II CTD repeat (Swiss Prot: P24928).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen a KLH-coupled synthetic phosphopeptide corresponding to the residues surrounding serine 5 of human RNA polymerase II CTD repeat (Swiss Prot: P24928)
Purification Affinity chromatography using phospho-peptide, and cross-adsorbed with the corresponding non-phosphopeptide

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript RNA polymerase II CTD repeat Antibody (Phospho-Ser5), pAb, Rabbit detects endogenous level of human RNA polymerase II CTD repeat protein. Predicted to react with mouse and rat RNA polymerase II CTD repeat according to sequence homology.
Positive Control: HEK293
Species Reactivity Human, Reactivity to other species is not tested yet.
Predicted Band Size 217 kD
Observed Band Size 217 kD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml
Other Applications: User optimized

  • RNA Polymerase II CTD Repeat Antibody (Phospho-Ser<sup>5</sup>), PAb, Rabbit
  • RNA Polymerase II CTD Repeat Antibody (Phospho-Ser<sup>5</sup>), PAb, Rabbit

    Western blot analysis of cell lysates using RNA polymerase II CTD repeat Antibody(Phospho-Ser5), pAb, Rabbit (GenScript, A01634, 2 µg/ml)
    The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 217 kD
    Observed Size: 217 kD


Cat. No.
Product Name
Price
A01633-40
RNA polymerase II CTD repeat Antibody (Phospho-Ser2), pAb, Rabbit - 40 ug
$99.00
 



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Cat. No. A01634
Size
Price
40 ug $99.00