|Synonyms||SNAIL Antibody, pAb, Rabbit; SNAI2 Antibody, pAb, Rabbit|
|Description||SNAl protein family is a transcriptional repressor which contains SNAl1 and SNAI2. SNAl protein binds to the E-cadherin promoter region to repress transcription during development. The binding of SNAI protein to integrin promoter sequences represses integrin expression and results in reduced cell adhesion. The tumor suppressor protein p53 induces SNAL expression in γ-irradiated cells. Deletion mutations in the corresponding SNAI gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism.
GenScript SNAI 1/2 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human SNAl1 (Swiss Prot: O95863) and human SNAI2 (Swiss Prot: O43623).
|Immunogen||KLH-coupled synthetic peptide from C-terminal of human SNAl1 (Swiss Prot: O95863) and human SNAI2 (Swiss Prot: O43623)|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript SNAI 1/2 Antibody, pAb, Rabbit detects endogenous levels of human SNAI 1/2. Predicted to react with mouse and rat SNAI2 according to sequence homology.
Positive Control: MCF-7, HEK293
|Species Reactivity||Human. Reactivity to other species is not tested yet.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/ml.
Other Applications: User optimized.
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
|Cat. No. A01641|