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Home » Gene Synthesis & Molecular Biology Services » CD45 Antibody, pAb, Rabbit

CD45 Antibody, pAb, Rabbit

Synonyms PTPRC Antibody, pAb, Rabbit;
Description CD45, also known as leukocyte-common antigen (LCA) is an integral membrane protein tyrosine phosphatase expressed on leukocyte cell surface. It suppresses JAK kinases and negatively regulates cytokine receptor signaling. CD45 is essential for activation of T and B cells by mediating cell-to-cell contacts and regulating protein-tyrosine kinases involved in signal transduction. CD45 is also involved in integrin-mediated adhesion and migration of immune cells. It can be used to identify undifferentiated hematopoietic tumors as an antigenic marker.
GenScript CD45 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human CD45 protein (Swiss Prot: P08575).
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen KLH-coupled synthetic peptide from C-terminal of human CD45 protein (Swiss Prot: P08575)
Purification Immunoaffinity chromatography

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScriptCD45 Antibody, pAb, Rabbit detects endogenous levels of human CD45 protein.
Positive Control: Jurkat
Species Reactivity Human. Reactivity to other species has not been tested yet.
Predicted Band Size 147 KD
Observed Band Size 147 KD
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/ml
Other Applications: user optimized

  • CD45 Antibody, PAb, Rabbit
  • CD45 Antibody, PAb, Rabbit

    Western blot analysis of cell lysates using CD45 Antibody, pAb, Rabbit (GenScript, A01655, 1 µg/ml)
    The signal was developed with IRDye™ 800 Conjugated Goat Anti-Rabbit IgG.
    Predicted Size: 147 KD
    Observed Size: 147 KD




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Cat. No. A01655
Size
Price
40 ug $99.00