PU.1 Antibody, pAb, Rabbit- GenScript, AAALAC International Accredited Animal Facility

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Overview

Synonyms	PU.1 Antibody, pAb, Rabbit; Spi1 antibody
Description	PU.1 known as Spi1 and Sfpi1, is an ETS-domain transcription factor essential for the development of myeloid and B-lymphoid cells. In myeloid cells, PU.1 may regulate transcription of both FMS and CD18/11B proteins that are central to the osteoclast phenotype. The failure to express PU.1 might preclude development of osteoclasts, leading to arrested bone resorption and osteopetrosis. PU.1 expression increases with the induction of osteoclastogenesis by either 1,25-dihydroxyvitamin D3 or dexamethasone which confirms the association between PU.1 and osteoclast differentiation. GenScript PU.1 Antibody, pAb, Rabbit is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human PU.1 protein (Swiss Prot: P17947).
Host Species	Rabbit
Antigen Species	Human
Conjugation	Unconjugated
Immunogen	KLH-coupled synthetic peptide from C-terminal of human PU.1 (Swiss Prot: P17947)
Purification	Immunoaffinity chromatography

Properties

Subclass	IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	GenScript PU.1 Antibody, pAb, Rabbit detects human PU.1 protein.
Species Reactivity	Human. Reactivity to other species has not been tested yet.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1-2 µg/ml Other Applications: user optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

Western blot analysis of extracts from HEK293 cells transfected with human PU.1 for 72hr using PU.1 Antibody, pAb, Rabbit (GenScript, A01692, 2 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 31 kD
Observed Size: 31 kD

Western blot analysis of human recombinant PU.1 fusion protein using PU.1 Antibody, pAb, Rabbit (GenScript, A01692, 2 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.
Predicted Size: 62 kD
Observed Size: 62 kD