SUMO-tag Antibody(4G11E9), mAb, Mouse Antibody

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Overview

Synonyms	SUMO Antibody;
Description	SUMO tags are emerging as a viable alternative for increasing both the expression and solubility of otherwise hard-to-express proteins. The SUMO tag can be cleanly excised using SUMO protease, which recognizes the conformation of SUMO protein rather than a specific sequence within SUMO. The SUMO system can be used effectively in both prokaryotic and eukaryotic systems. GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is suitable for detecting fusion proteins that contain a SUMO-tag. GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with recombinant yeast SUMO protein.
Host Species	Mouse
Conjugation	Unconjugated
Immunogen	Purified recombinant yeast SUMO protein
Purification	Protein A affinity column

Properties

Subclass	IgG2a k
Clone ID	4G11E9
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	GenScript SUMO-tag Antibody (4G11E9), mAb, Mouse specifically reacts with fusion proteins containing SUMO epitope tags. Positive Control: Yeast cell lysates
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1 µg/ml
Other Applications: user optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

Western blot analysis of Yeast cell lysates using SUMO-tag Antibody (4G11E9), mAb, Mouse (GenScript, A01693, 1 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 12 kD
Observed Size: 12 kD

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Zhang Y, et al. The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the of human DNA polymerase α-primase complex. J Biol Chem. 2014June;

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