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Catalog Products » Antibodies » Epitope Tag Antibodies » GFP Antibody, pAb, Chicken

GFP Antibody, pAb, Chicken

*This product has been discontinued!*
Synonyms Green Fluorescent Protein antibody;
Description Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.
GenScript GFP Antibody, pAb, Chicken is developed in chicken using purified recombinant full-length GFP protein.
Host Species Chicken
Conjugation Unconjugated
Immunogen purified recombinant full-length GFP protein
Purification Immunoaffinity chromatography

Subclass IgY
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript GFP Antibody, pAb, Chicken specifically reacts with fusion proteins containing GFP epitope tag. This antibody recognizes all variants of GFP protein such as eGFP, AcGFP1 and cGFP, and a variety of variants of RFP protein such as DsRed, DeRed2, mCherry and tdTomato. It slightly recognizes mOrange protein.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
Other Applications: user optimized

Cat. No. Product Name Quantity Price
THE™ GFP Antibody, pAb, Rabbit - 100 ug
GFP Antibody, pAb, Rabbit - 40 ug
JC McIntyre., et al. Gene Therapy Rescues Cilia Defects And Restores Olfactory Function In A Mammalian Ciliopathy Model. Nat Med. 2012 Sep;18(9):1423 - 8.

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