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Catalog Products » Antibodies » IVD Antibodies » TSH Antibody (2B9H4), mAb, Mouse

TSH Antibody (2B9H4), mAb, Mouse

TSH Antibody (2B9H4), MAb, Mouse

Cross-reactivity of TSH monoclonal antibodies by Indirect ELISA:
General conditions:
1. Microplate was incubated with human TSH, or human HCG respectively, followed by 3 washing cycles.
2. Incubation with mouse anti-TSH antibody followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

TSH Antibody (2B9H4), MAb, Mouse

Antibody pairs analysis of TSH monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was incubated with a capture antibody against TSH, followed by 3 washing cycles.
2. Incubation with TSH followed by 3 washing cycles.
3. Incubation with peroxidase conjugated detection antibody against TSH, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

Synonyms FSHA antibody;
Description Thyrotropin-stimulating hormone (TSH) is a noncovalently linked glycoprotein heterodimer and is part of a family of pituitary hormones containing a common alpha subunit and a unique beta subunit that confers specificity. Free alpha and beta subunits have essentially no biological activity. TSH (Thyroid stimulating hormone) is secreted from cells in the anterior pituitary and it is indispensable for the control of thyroid structure and metabolism. Free alpha and beta subunits have essentially no biological activity.
GenScript TSH Antibody (2B9H4), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified TSH from human pituitary.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen purified TSH from human pituitary
Purification Protein A affinity column

Subclass IgG2a κ
Clone ID 2B9H4
Concentration 0.5mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Products of 1mg and 5mg size are provided in liquid form.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript TSH Antibody (2B9H4), mAb, Mouse detects endogenous levels of human TSH and does not cross-react with human HCG.
Species Reactivity Human. Reactivity to other species is not tested yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 0.5-10 µg/ml
ELISA Detection: 0.05-0.2 µg/ml
Recommended antibody pairing for sandwich immunoassay:
These antibodies are perfect choice for in vitro diagnostic assay development. And they are prepared for non-clinical research use only. The recommended pairs are based on our laboratory results.
Capture
Detection A01709
(1H4G9)
A01710
(2D10F6)
A01711
(2B9H4)
A01712
(26A7D2)
A01713
(28F7B8)
A01714
(3B12D11)
A01709
(1H4G9)
- - +++ - -
A01710
(2D10F6)
- - ++ - -
A01711
(2B9H4)
- - - + -
A01712
(26A7D2)
+ + + - ++
A01713
(28F7B8)
- - +++ - -
A01714
(3B12D11)
- - - - -
The above Data was achieved by Sandwich ELISA. ‘+’ means reaction and ‘-‘means no reaction.
The number of ‘+’ represents reaction intensity.
Other Applications: user optimized

TSH Antibody (2B9H4), MAb, Mouse
TSH Antibody (2B9H4), MAb, Mouse

Cross-reactivity of TSH monoclonal antibodies by Indirect ELISA:
General conditions:
1. Microplate was incubated with human TSH, or human HCG respectively, followed by 3 washing cycles.
2. Incubation with mouse anti-TSH antibody followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

TSH Antibody (2B9H4), MAb, Mouse
TSH Antibody (2B9H4), MAb, Mouse

Antibody pairs analysis of TSH monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was incubated with a capture antibody against TSH, followed by 3 washing cycles.
2. Incubation with TSH followed by 3 washing cycles.
3. Incubation with peroxidase conjugated detection antibody against TSH, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.




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Cat. No. A01711
Size
Price
5 mg $975.00
40 ug $99.00
1 mg $230.00
24 hour
 
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