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Catalog Products » Antibodies » IVD Antibodies » Insulin Antibody (5A6A4), mAb, Mouse

Insulin Antibody (5A6A4), mAb, Mouse

Insulin Antibody (5A6A4), MAb, Mouse

Cross-reactivity of Insulin monoclonal antibodies by Indirect ELISA:
General conditions:
1. Microplate was coated with insulin, proinsulin or C-peptide respectively, followed by 3 washing cycles.
2. Incubation with mouse anti-insulin antibody followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

Insulin Antibody (5A6A4), MAb, Mouse

Antibody pairs analysis of Insulin monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was coated with a capture antibody against insulin, followed by 3 washing cycles.
2. Incubation with insulin followed by 3 washing cycles.
3. Incubation with peroxidase conjugated detection antibody against insulin, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

Synonyms ILPR antibody;
Description Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. Proinsulin is the prohormone precursor to insulin made in pancreas. It is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-Peptide. The C-Peptide is abstracted from the center of the proinsulin sequence; the two other ends (α and β chains) remain connected by disulfide bonds. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B cell origin such as insulinoma.
GenScript Insulin Antibody (5A6A4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant Insulin expressed in yeast.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen human recombinant Insulin expressed in yeast
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2b κ
Clone ID 5A6A4
Concentration 0.5mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Products of 1mg and 5mg size are provided in liquid form.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript Insulin Antibody (5A6A4), mAb, Mouse detects human Insulin and it has no cross-reactivity with Proinsulin and C-peptide.
Species Reactivity Human. Reactivity to other species is not tested yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA Capture: 1-10 µg/ml
ELISA Detection: 0.05-0.2 µg/ml
Recommended antibody pairing for sandwich immunoassay:

These antibodies are perfect choice for in vitro diagnostic assay development. And they are prepared for non-clinical research use only. The recommended pairs are based on our laboratory results.
Capture
Detection A01715
(6E9F1)
A01716
(5A6A4)
A01715
(6E9F1)
-
A01716
(5A6A4)
+++
The above Data was achieved by Sandwich ELISA. ‘+’ means reaction and ‘-‘means no reaction.
The number of ‘+’ represents reaction intensity.
Other applications: user-optimized

Insulin Antibody (5A6A4), MAb, Mouse
Insulin Antibody (5A6A4), MAb, Mouse

Cross-reactivity of Insulin monoclonal antibodies by Indirect ELISA:
General conditions:
1. Microplate was coated with insulin, proinsulin or C-peptide respectively, followed by 3 washing cycles.
2. Incubation with mouse anti-insulin antibody followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.

Insulin Antibody (5A6A4), MAb, Mouse
Insulin Antibody (5A6A4), MAb, Mouse

Antibody pairs analysis of Insulin monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was coated with a capture antibody against insulin, followed by 3 washing cycles.
2. Incubation with insulin followed by 3 washing cycles.
3. Incubation with peroxidase conjugated detection antibody against insulin, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.




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Cat. No. A01716
Size
Price
5 mg $830.00
100 ug $199.00
1 mg $206.00
24 hour
 
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