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Catalog Products » Antibodies » Primary Antibodies » Control Antibodies » β-tubulin Antibody (2G7D4), mAb, Mouse

β-tubulin Antibody (2G7D4), mAb, Mouse

β-tubulin Antibody (2G7D4), MAb, Mouse

Western blot analysis of cell and tissue lysates using β-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, A01717, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
Predicted Size: 50 kD
Observed Size: 50 kD

β-tubulin Antibody (2G7D4), MAb, Mouse

Flow cytometric analysis of CHO cells using β-tubulin Antibody (2G7D4), mAb, Mouse (Green) or with an isotype control antibody (GenScript, A01007; open histogram) (black)
followed by FITC conjugated anti-mouse IgG.

β-tubulin Antibody (2G7D4), MAb, Mouse

Immunohistochemistry analysis of human colon tissue slide (Paraffin embedded) using β-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, Cat.No. A01717, 15 µg/ml)

Synonyms Beta tubulin antibody;
Description Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (α) and beta (β) chains. β-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots.
GenScript β-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length β-tubulin protein.
Host Species Mouse
Conjugation Unconjugated
Immunogen purified human recombinant full-length β-tubulin protein
Purification Protein A affinity column

Subclass IgG1,K
Clone ID 2G7D4
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript β-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish β-tubulin. This product has not yet been tested in other species.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
Flow cytometry:1 µg for 1 x 106 cells
Immunohistochemistry: 10-20 µg/ml


  • β-tubulin Antibody (2G7D4), MAb, Mouse
  • β-tubulin Antibody (2G7D4), MAb, Mouse

    Western blot analysis of cell and tissue lysates using β-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, A01717, 1 µg/ml)
    The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
    Predicted Size: 50 kD
    Observed Size: 50 kD

  • β-tubulin Antibody (2G7D4), MAb, Mouse
  • β-tubulin Antibody (2G7D4), MAb, Mouse

    Flow cytometric analysis of CHO cells using β-tubulin Antibody (2G7D4), mAb, Mouse (Green) or with an isotype control antibody (GenScript, A01007; open histogram) (black)
    followed by FITC conjugated anti-mouse IgG.

  • β-tubulin Antibody (2G7D4), MAb, Mouse
  • β-tubulin Antibody (2G7D4), MAb, Mouse

    Immunohistochemistry analysis of human colon tissue slide (Paraffin embedded) using β-tubulin Antibody (2G7D4), mAb, Mouse (GenScript, Cat.No. A01717, 15 µg/ml)


Cat. No.
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THETM alpha Tubulin Antibody [Biotin], mAb, Mouse - 100 ug
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THETM alpha Tubulin Antibody [HRP], mAb, Mouse - 100 ug
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THETM alpha Tubulin Antibody, mAb, Mouse - 100 ug
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β-Tubulin III Antibody, pAb, Rabbit - 40 ug
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Infanger DW., et al. Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche. Cancer Res. 2013 Dec;73(23):7079-89.

Zhenxun Wang., et al. Exon-Centric Regulation Of Pyruvate Kinase M Alternative Splicing Via Mutually Exclusive Exons. J Mol Cell Biol. 2012 Apr;4(2):79-87.

Huang LH, et al. Acyl-Coenzyme A:cholesterol Acyltransferase 1: Significance of Single Nucleotide Polymorphism at Residue 526 and Role of Proline 347 near the Fifth Transmembrane Domain. FEBS J. 2014Feb.;

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Cat. No. A01717
Size
Price
40 ug $99.00