• English
  • Sign In
  • Contact Us
×
Support » Promotions » Product Promotions » Free Samples » THETM V5 Tag Antibody, mAb, Mouse

THETM V5 Tag Antibody, mAb, Mouse

Synonyms THETM V5 Tag Antibody, mAb, Mouse;
Description The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
THETM V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Immunogen V5 tag peptide conjugated to KLH
Fusion Partner Fusion of SP2/0-Ag14 myeloma and B-lymphocytes
Purification Protein A affinity column

Subclass IgG2a K
Clone ID 4C12E11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM V5 Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/ml
Western blot: 0.2-1 µg/ml
Immunoprecipitation: 1-5 µg/ml
FACS: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml

  • THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse
  • THE<sup>TM</sup> V5 Tag Antibody, MAb, Mouse

    Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using
    THETM V5 Tag Antibody, mAb, Mouse (GenScript, A01724) (black and red respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.


Cat. No. Product Name Quantity Price
A01803-100
THETM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$263.00
A01804-100
THETM V5 Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$263.00
A01805-100
THETM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$263.00
 


Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A01724S
Size Price
10 μg $0.00