|Synonyms||C-peptide Antibody (8G1D12), mAb, Mouse;|
|Description||C-peptide serves as an important linker between A-chain and B-chain of insulin and facilitates the efficient assembly, folding, and processing of insulin in the endoplasmic reticulum. Equimolar amounts of C-peptide and insulin are stored in secretory granules of the pancreatic beta cells and both are eventually released to the portal circulation. The sole interest in C-peptide was as a marker of insulin secretion. Newly diagnosed diabetes patients often get their C-peptide levels measured as a means of distinguishing type 1 and type 2 diabetes. C-peptide is also used for determining the possibility of gastrinomas associated with Multiple Endocrine Neoplasm syndromes (MEN 1).
GenScript Human C-Peptide Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human C-peptide conjugated to KLH.
|Immunogen||human C-peptide conjugated to KLH.|
|Purification||Protein A affinity column|
|Concentration||0.5mg/ml，in PBS buffer, pH 7.4, containing 0.02% sodium azide.|
|Specificity||C-peptide monoclonal antibodies (Clone 3E8D9, 8E10D3, 4C2F10 and 8G1D12) recognize human C-peptide and don’t cross-react with proinsulin and insulin.|
|Storage||For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
Antibody pairs analysis of C-peptide monoclonal antibodies by Sandwich ELISA:
1. Microplate was coated with a capture antibody against C-peptide, followed by 3 washing cycles.
2. Incubation with C-peptide followed by 3 washing cycles.
3. Incubation with Biotin conjugated detection antibody against C-peptide, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles.
Colorimetric determination of bound peroxidase activity.