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Catalog Products » Antibodies » IVD Antibodies » C-peptide Antibody (8E10D3), mAb, Mouse

C-peptide Antibody (8E10D3), mAb, Mouse

Synonyms C-peptide Antibody (8E10D3), mAb, Mouse;
Description C-peptide serves as an important linker between A-chain and B-chain of insulin and facilitates the efficient assembly, folding, and processing of insulin in the endoplasmic reticulum. Equimolar amounts of C-peptide and insulin are stored in secretory granules of the pancreatic beta cells and both are eventually released to the portal circulation. The sole interest in C-peptide was as a marker of insulin secretion. Newly diagnosed diabetes patients often get their C-peptide levels measured as a means of distinguishing type 1 and type 2 diabetes. C-peptide is also used for determining the possibility of gastrinomas associated with Multiple Endocrine Neoplasm syndromes (MEN 1).
GenScript Human C-Peptide Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human C-peptide conjugated to KLH.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen human C-peptide conjugated to KLH.
Purification Protein A affinity column

Subclass IgG2b,κ
Clone ID 8E10D3
Concentration 0.5mg/ml,in PBS buffer, pH 7.4, containing 0.02% sodium azide.
Specificity C-peptide monoclonal antibodies (Clone 3E8D9, 8E10D3, 4C2F10 and 8G1D12) recognize human C-peptide and don’t cross-react with proinsulin and insulin.
Storage For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.


C-peptide Antibody (8E10D3), MAb, Mouse
C-peptide Antibody (8E10D3), MAb, Mouse

Antibody pairs analysis of C-peptide monoclonal antibodies by Sandwich ELISA:
General procedures:
1. Microplate was coated with a capture antibody against C-peptide, followed by 3 washing cycles.
2. Incubation with C-peptide followed by 3 washing cycles.
3. Incubation with Biotin conjugated detection antibody against C-peptide, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles.
Colorimetric determination of bound peroxidase activity.




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Cat. No. A01727
Size
Price
5 mg $1385.00
1 mg $333.00
24 hour
 
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