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Cross‐reactivity of PSA monoclonal antibodies by Indirect ELISA:
1. Microplate was coated with Proinsulin, Insulin or C‐peptide respectively, followed by 3 washing cycles.
2. Incubation with mouse anti‐Proinsulin antibody followed by 3 washing cycles.
3. Incubation with goat anti‐mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.
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