THETM V5 Tag Antibody [HRP], mAb, Mouse Antibody

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Overview

Synonyms	THE^TM V5 Tag Antibody [HRP], mAb, Mouse ;
Description	V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope. GenScript THE^TM V5 Tag Antibody [HRP], mAb, Mouse is THE^TM V5 Tag Antibody, mAb, Mouse (A01724) conjugated with Horseradish peroxidase (HRP). The antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Host Species	Mouse
Conjugation	Peroxidase (Horseradish)
Immunogen	V5 tag peptide conjugated to KLH
Purification	Protein A affinity column

Properties

Subclass	IgG2a к
Clone ID	4C12E11
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.01% thimerosal.
Reconstitution	Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity	THE^TM V5 Tag Antibody [HRP], mAb, Mouse recognizes C-terminal tagged fusion proteins, and it slightly recognizes N-terminal V5 tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.1 µg/ml.
Western blot: 0.2-1 µg/ml.

Examples

THE<sup>TM</sup> V5 Tag Antibody [HRP], MAb, Mouse

Western blot analysis of C-terminal V5 tagged fusion protein using THE^TM V5 Tag Antibody [HRP], mAb, Mouse (A01733, 1 µg/ml)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221)

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