Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ V5 Tag Antibody [Biotin], mAb, Mouse
THE™ V5 Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of N-terminal V5 tagged fusion protein and C-terminal tagged fusion protein using THETM V5 Tag Antibody [Biotin], mAb, Mouse (A01735, 1 µg/ml)
The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)

THE™ V5 Tag Antibody [Biotin], mAb, Mouse

THE™ V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
A01735
$245.00

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Overview
Specificity THE™ V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
Host Species Mouse
Immunogen V5 tag peptide conjugated to KLH
Conjugate Biotin

Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.005-0.05 µg/ml
Western Blot 0.2-1 µg/ml

Properties
Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 4C12E11

Examples
  • THE™ V5 Tag Antibody [Biotin], MAb, Mouse
    • THE™ V5 Tag Antibody [Biotin], MAb, Mouse

    Western blot analysis of N-terminal V5 tagged fusion protein and C-terminal tagged fusion protein using THETM V5 Tag Antibody [Biotin], mAb, Mouse (A01735, 1 µg/ml)
    The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)


Background
Target Background V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).GenScript THE™ V5 Tag Antibody [Biotin], mAb, Mouse is THE™ V5 Tag Antibody (A01724) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Synonyms THE™ V5 Tag Antibody [Biotin], mAb, Mouse ;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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