THETM V5 Tag Antibody [Biotin], mAb, Mouse
| Synonyms | THETM V5 Tag Antibody [Biotin], mAb, Mouse ; |
| Description | V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). GenScript THETM V5 Tag Antibody [Biotin], mAb, Mouse is THETM V5 Tag Antibody (A01724) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins. |
| Host Species | Mouse |
| Conjugation | Biotin |
| Immunogen | V5 tag peptide conjugated to KLH |
| Purification | Protein A affinity column |
| Subclass | IgG2a K |
| Clone ID | 4C12E11 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. |
| Specificity | THETM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.005-0.05 µg/ml Western blot: 0.2-1 µg/ml |
![THE<sup>TM</sup> V5 Tag Antibody [Biotin], MAb, Mouse](/product/documents/down?doc_name=13653_20110310224009.JPG&file=filecenter/document/13653_20110310224009.JPG "THE<sup>TM</sup> V5 Tag Antibody [Biotin], MAb, Mouse")
Western blot analysis of N-terminal V5 tagged fusion protein and C-terminal tagged fusion protein using THETM V5 Tag Antibody [Biotin], mAb, Mouse (A01735, 1 µg/ml)
The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01805-100 |
THETM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug |
$263.00 |
|
A01804-100 |
THETM V5 Tag Antibody [iFluor 555], mAb, Mouse - 100 ug |
$263.00 |
|
A01803-100 |
THETM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug |
$263.00 |
|
A01733-100 |
THETM V5 Tag Antibody [HRP], mAb, Mouse - 100 ug |
$239.00 |
|
A01724-100 |
THETM V5 Tag Antibody, mAb, Mouse - 100 ug |
$219.00 |
|
A01724-200 |
THETM V5 Tag Antibody, mAb, Mouse - 200 ug |
$395.00 |
|
A00877-40 |
V5-tag Antibody [HRP], pAb, Rabbit - 40 ug |
$99.00 |
|
A00623-40 |
V5-tag Antibody, pAb, Rabbit - 40 ug |
$99.00 |
For more documents, please visit "Technical Support".
| Cat. No. A01735 | ||
|---|---|---|
| Size | Price | |
| 100 ug | $239.00 | |
