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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM V5 Tag Antibody [Biotin], mAb, Mouse

THETM V5 Tag Antibody [Biotin], mAb, Mouse

Synonyms THETM V5 Tag Antibody [Biotin], mAb, Mouse ;
Description V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
GenScript THETM V5 Tag Antibody [Biotin], mAb, Mouse is THETM V5 Tag Antibody (A01724) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of V5 Tagged fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen V5 tag peptide conjugated to KLH
Purification Protein A affinity column

Subclass IgG2a K
Clone ID 4C12E11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM V5 Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal V5 tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.005-0.05 µg/ml
Western blot: 0.2-1 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> V5 Tag Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> V5 Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of N-terminal V5 tagged fusion protein and C-terminal tagged fusion protein using THETM V5 Tag Antibody [Biotin], mAb, Mouse (A01735, 1 µg/ml)
The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)


Cat. No.
Product Name
Size
Price
Quantity
A00623-100 V5-tag Antibody, pAb, Rabbit
100 ug
$199.00
A01724-40 THETM V5 Tag Antibody, mAb, Mouse
40 ug
$119.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 ug
$219.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 ug
$395.00
A01733-100 THETM V5 Tag Antibody [HRP], mAb, Mouse
100 ug
$239.00
A00877-100 V5-tag Antibody [HRP], pAb, Rabbit
100 ug
$199.00
A01803-100 THETM V5 Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$263.00
A01804-100 THETM V5 Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$263.00
A01805-100 THETM V5 Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$263.00
 


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Cat. No. A01735
Size
Price
100 ug $239.00
 
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