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Catalog Products » Other Products » THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse

THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse

Synonyms THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse;
Description Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
GenScript THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with biotin. The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK (Strep II tag) tagged fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen NWSHPQFEK peptide conjugated to KLH
Purification Protein A affinity column

Subclass IgG1 Κ
Clone ID 5A9F9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
ELISA: 0.001-0.002 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> NWSHPQFEK Tag Antibody [Biotin], MAb, Mouse
THE<sup>TM</sup> NWSHPQFEK Tag Antibody [Biotin], MAb, Mouse

Western blot analysis of N-terminal and C-terminal Strep II tagged fusion protein using THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse (GenScript, A01737, 1 µg/ml)
The signal was developed with Streptavidin-HRP conjugate (GenScript, 1:5,000, M00091) and LumiSensor THETM HRP Substrate Kit (GenScript, L00221)


Cat. No.
Product Name
Size
Price
Quantity
A00626-40 NWSHPQFEK Antibody, pAb, Rabbit
40 ug
$99.00
A01732-100 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
100 ug
$319.00
A01732-200 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
200 ug
$579.00
A01736-100 THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse
100 ug
$349.00
A01742-40 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
40 ug
$154.00
A01742-100 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
100 ug
$349.00
A01742-200 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
200 ug
$637.00
A00875-100 NWSHPQFEK Antibody [HRP], pAb, Rabbit
100 ug
$199.00
 
Sangmin L, et al. Novel somatic single nucleotide variants within the RNA binding protein hnRNP A1 in multiple sclerosis patients. F1000Res. 2014June;

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Cat. No. A01737
Size
Price
100 ug $349.00
 
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