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Catalog Products » Antibodies » Epitope Tag Antibodies » Avi Tag Antibody, mAb, Mouse

Avi Tag Antibody, mAb, Mouse

Avi Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (a) and Avi fusion protein transfeced CHO cells (b) using Avi Antibody, mAb, Mouse (GenScript, A01738). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of Avi tagged protein transfeced CHO cells using Avi Tag Antibody, mAb, Mouse (GenScript, A01738) and Mouse IgG Control (GenScript, A01007).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse

Western blot analysis of C-terminal Avi tagged fusion protein using Avi Tag Antibody, mAb, Mouse (GenScript, A01738, 0.2 µg/ml).
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse

ELISA analysis of N-terminal and C-terminal Avi tagged fusion proteins using Avi Tag Antibody, mAb, Mouse (GenScript, A01738).

Avi Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or AVI fusion gene transfected CHO cells using
AVI Tag Antibody, mAb, Mouse (GenScript, A01738) (black and purple respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Synonyms BAP Tag antibody;
Description Avi tag is a biotin-acceptor peptide, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Avi Tag Antibody is a useful tool in analysis of Avi fusion proteins.
Host Species Mouse
Conjugation Unconjugated
Immunogen Avi peptide conjugated to KLH
Fusion Partner fusion of SP2/0-Ag14 myeloma and mouse B-lymphocytes
Purification Protein A affinity column

Subclass IgG2a,κ
Clone ID 1D11D10
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript Avi Tag Antibody, mAb, Mouse recognizes C-terminal Avi tagged fusion proteins and slightly recognizes N-terminal Avi tagged fusion proteins in Western Blot.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
FACS: 1-3 µg for 1 x 106 cells
ICC/IF: 1-3 µg/ml
ELISA: 0.02-0.05 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Avi Tag Antibody, MAb, Mouse
Avi Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (a) and Avi fusion protein transfeced CHO cells (b) using Avi Antibody, mAb, Mouse (GenScript, A01738). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse
Avi Tag Antibody, MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of Avi tagged protein transfeced CHO cells using Avi Tag Antibody, mAb, Mouse (GenScript, A01738) and Mouse IgG Control (GenScript, A01007).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse
Avi Tag Antibody, MAb, Mouse

Western blot analysis of C-terminal Avi tagged fusion protein using Avi Tag Antibody, mAb, Mouse (GenScript, A01738, 0.2 µg/ml).
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Avi Tag Antibody, MAb, Mouse
Avi Tag Antibody, MAb, Mouse

ELISA analysis of N-terminal and C-terminal Avi tagged fusion proteins using Avi Tag Antibody, mAb, Mouse (GenScript, A01738).

Avi Tag Antibody, MAb, Mouse
Avi Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or AVI fusion gene transfected CHO cells using
AVI Tag Antibody, mAb, Mouse (GenScript, A01738) (black and purple respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.




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Cat. No. A01738
Size
Price
100 ug $199.00
 
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