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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse

THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse

Synonyms THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse;
Description Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep II tag) is a nine amino acid peptide with high specificity and affinity towards streptavidin.
GenScript THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse is THE™ NWSHPQFEK Tag Antibody (A01732) conjugated with Horseradish peroxidase (HRP). The conjugated antibody is suitable for detecting the expression level of NWSHPQFEK Tagged fusion proteins.
Host Species Mouse
Conjugation Peroxidase (Horseradish)
Immunogen NWSHPQFEK peptide conjugated to KLH
Purification Protein A affinity column

Subclass IgG1 Κ
Clone ID 5A9F9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse recognizes N-terminal and C-terminal Strep II tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/ml
ELISA: 0.001-0.002 µg/ml

  • THE™ NWSHPQFEK Tag Antibody [HRP], MAb, Mouse
  • THE™ NWSHPQFEK Tag Antibody [HRP], MAb, Mouse

    Western blot analysis of N-terminal Strep II tagged fusion protein and C-terminal Step II tagged protein using THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse (GenScript, A01742, 1 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)

Cat. No. Product Name Quantity Price
THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 100 ug
THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse - 100 ug
THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse - 100 ug
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 100 ug
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 200 ug
NWSHPQFEK Antibody [HRP], pAb, Rabbit - 40 ug
NWSHPQFEK Antibody, pAb, Rabbit - 40 ug
Remco Stam., et al. Identification and Characterisation CRN Effectors in Phytophthora capsici Shows Modularity and Functional Diversity. PLoS One. 2013 Mar;8(3):e59517.

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Cat. No. A01742
Size Price
100 ug $349.00