|Synonyms||Human HBsAg antibody (1C10E2), mAb, Mouse;|
|Description||Hepatitis B virus (HBV) is an enveloped, double-stranded DNA virus. Infection with HBV induces a spectrum of clinical manifestations ranging from mild, inapparent disease to fulminant hepatitis, severe chronic liver diseases. Hepatitis B surface antigen or HBsAg is the most important protein of the envelope of Hepatitis B Virus. The surface antigen contains the determinant “a”, common to all known viral subtypes and immunologically distinguished in two distinct subgroups (ay and ad). The serological detection of HBsAg is a powerful method for the diagnosis and prevention of HBV infection and ELISA has become an extensively used analytical system for screening of blood donors and clinical diagnosis of HBV in infected individuals.
GenScript Human HBsAg Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human Hepatitis B Surface Antigen.
|Immunogen||Purified human Hepatitis B Surface Antigen|
|Purification||Protein A affinity column|
|Specificity||Human HBsAg monoclonal antibodies (1C10E2, 1G1A10 and 3G9F6) recognize Hepatitis B Surface Antigen.|
|Storage||For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
HBsAg monoclonal antibodies by Indirect ELISA:
1. Microplate was incubated with HBsAg, followed by 3 washing cycles.
2. Incubation with mouse anti-HBsAg followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.