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HBsAg monoclonal antibodies by Indirect ELISA:
General conditions:1. Microplate was incubated with HBsAg, followed by 3 washing cycles.
2. Incubation with mouse anti-HBsAg followed by 3 washing cycles.
3. Incubation with goat anti-mouse lgG conjugated to peroxidase, followed by 3 washing cycles.
4. Colorimetric determination of bound peroxidase activity.
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