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Catalog Products » Antibodies » IVD Antibodies » Human NGAL Antibody (5A9D12), mAb, Mouse

Human NGAL Antibody (5A9D12), mAb, Mouse

Synonyms Human NGAL Antibody (5A9D12), mAb, Mouse;
Description NGAL (Neutrophil gelatinase-associated lipocalin, also known as lipocalin-2) is a small protein found in renal tubules. And its expression is dramatically increased in ischemic or nephrotoxic injury. It has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis. NGAL is expressed in variety of tumor types including breast, ovary and pancreatic carcinomas.
Human NGAL Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human recombinant NGAL (NP_005555.2) expressed in mammalian cells.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen human recombinant NGAL expressed in mammalian cells
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 5A9D12
Concentration 0.5mg/ml,in PBS buffer, pH 7.4, containing 0.02% sodium azide.
Specificity NGAL monoclonal antibodies (5A9D12, 4C10A7) recognize human NGAL protein.
Storage For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.


Human NGAL Antibody (5A9D12), MAb, Mouse
Human NGAL Antibody (5A9D12), MAb, Mouse

Antibody pairs analysis of NGAL monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was incubated with a capture antibody (clone 5A9D12) against NGAL and blocked with PBS containing1% BSA, followed by 3 washing cycles.
2. Incubation with NGAL protein followed by 3 washing cycles.
3. Incubation with Biotin labeled detection antibody (clone 4C10A7) against NGAL, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles.
5. Colorimetric determination of bound peroxidase activity.




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Cat. No. A01782
Size
Price
5 mg $1678.00
1 mg $395.00
24 hour
 
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