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Antibody pairs analysis of NGAL monoclonal antibodies by Sandwich ELISA:
1. Microplate was incubated with a capture antibody (clone 5A9D12) against NGAL and blocked with PBS containing1% BSA, followed by 3 washing cycles.
2. Incubation with NGAL protein followed by 3 washing cycles.
3. Incubation with Biotin labeled detection antibody (clone 4C10A7) against NGAL, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles.
5. Colorimetric determination of bound peroxidase activity.
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