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Catalog Products » Antibodies » IVD Antibodies » Human AFP Antibody (1D9H5), mAb, Mouse

Human AFP Antibody (1D9H5), mAb, Mouse

Synonyms Human AFP Antibody (1D9H5), mAb, Mouse;
Description AFP (Alpha-Feto Protein) is a glycoprotein with molecular weight of approximately 70 kDa. It is a major protein in developing fetus and decreases to lower level after birth. In healthy adults, less than 20 ng/ml of AFP is found in the serum. Serum AFP elevates when hepatocellular carcinoma or testicular germ cell tumors occurs. Therefore, it is a useful marker in diagnosing hepatocellular carcinoma and germ cell tumors. In addition, for pregnant women, the AFP concentration is 10-150 ng/ml in the blood. High levels of AFP can indicate a neural tube defect of the fetus, such us spina bifida.
GenScript Human AFP Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with AFP protein purified from human hepatocellular carcinoma (Swiss-Prot: P02771).
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen AFP protein purified from human hepatocellular carcinoma.
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 1D9H5
Concentration 0.5mg/ml,in PBS buffer, pH 7.4, containing 0.02% sodium azide.
Specificity AFP monoclonal antibodies (1D9H5, 2E1C10 and 3B6G7) recognize human AFP protein.
Sensitivity The limit of detection for antibody pairs is less than 20 pg/ml.
Storage For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

These antibodies are perfect choice for in vitro diagnostic assay development. They are prepared for non-clinical research use only. The recommended pairs are based on our laboratory results.
ELISA Capture: 1-10 μg/ml
ELISA Detection: 0.05-0.2 μg/ml
These antibodies are perfect choice for in vitro diagnostic assay development. And they are prepared for non-clinical research use only. The recommended pairs are based on our laboratory results.
Capture
Detection A01788
(1D9H5)
A01789
(2E1C10)
A01790
(3B6G7)
A01788
(1D9H5)
- +
A01789
(2E1C10)
+ -
A01790
(3B6G7)
- -
The above Data was achieved by Sandwich ELISA. ‘+’ means reaction and ‘-‘means no reaction.
The number of ‘+’ represents reaction intensity.


Human AFP Antibody (1D9H5), MAb, Mouse
Human AFP Antibody (1D9H5), MAb, Mouse

Antibody pairs analysis of AFP monoclonal antibodies by Sandwich ELISA:
General conditions:
1. Microplate was incubated with a capture antibody against AFP and blocked with PBS containing1% BSA, followed by 3 washing cycles.
2. Incubation with AFP protein followed by 3 washing cycles.
3. Incubation with Biotin labeled detection antibody against AFP, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles.
5. Colorimetric determination of bound peroxidase activity.




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Cat. No. A01788
Size
Price
5 mg $740.00
1 mg $175.00
24 hour
 
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