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Antibody pairs analysis of free PSA monoclonal antibodies by Sandwich ELISA:
1. Microplate was incubated with a capture antibody against free PSA and blocked with PBS containing1% BSA, followed by 3 washing cycles. 2. Incubation with free PSA protein followed by 3 washing cycles. 3. Incubation with Biotin labeled detection antibody against free PSA, followed by 3 washing cycles.
4. Incubation with Streptavidin-HRP, followed by 3 washing cycles. 5. Colorimetric determination of bound peroxidase activity.
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