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Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ His Tag Antibody [iFluor 647], mAb, Mouse

THE™ His Tag Antibody [iFluor 647], mAb, Mouse

THE™ His Tag Antibody [iFluor 647], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or His-tagged protein transfected CHO cells (Red)
using THETM His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, 4 µg/ml).

Specificity THE™ His Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal His-tagged proteins.
Host Species Mouse
Immunogen A synthetic peptide HHHHHH coupled to KLH
Conjugate iFluor 647

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Immunofluorescent staining  1-4 µg/ml
Flow cytometry  1-4 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG1,κ
Clonality Monoclonal
Clone ID 6G2A9

  • THE™ His Tag Antibody [iFluor 647], MAb, Mouse
  • THE™ His Tag Antibody [iFluor 647], MAb, Mouse

    Flow cytometric analysis of non-transfected CHO cells (Black) or His-tagged protein transfected CHO cells (Red)
    using THETM His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, 4 µg/ml).


Target Background The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.THE™ His Tag Antibody [iFluor 647], mAb, Mouse is THE™ His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Synonyms THE™ His Tag Antibody [iFluor 647], mAb, Mouse; His Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated His antibody; 647 conjugated His monoclonal antibody

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
L00440w
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
$179.00
L00440c
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
$179.00
L00440b
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
$179.00
L00439-1
Anti-His Affinity Resin - 1 ml Resin (Total 2 ml)
$399.00
L00439-5
Anti-His Affinity Resin - 5 ml Resin (Total 10 ml)
$1599.00
L00436
His Tag ELISA Detection Kit - 1 kit
$298.00
A01801-100
THE™ His Tag Antibody [iFluor 555], mAb, Mouse - 100 μg
$450.00
A01800-100
THE™ His Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
$450.00
A01620
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 μg
$410.00
A00613
THE™ His Tag Antibody [Biotin], mAb, Mouse - 100 μg
$410.00
A00612
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 μg
$410.00
A00205-40
His-tag antibody [Biotin], pAb, Rabbit - 40 μg
$99.00
A00186-100
THE™ His Tag Antibody, mAb, Mouse - 100 μg
$375.00
A00174-40
His-tag Antibody, pAb, Rabbit - 40 μg
$99.00
 




For more documents, please visit "Technical Support".

Cat. No. A01802
Size Price
100 μg $450.00