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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM His Tag Antibody [iFluor 647], mAb, Mouse

THETM His Tag Antibody [iFluor 647], mAb, Mouse

Synonyms THETM His Tag Antibody [iFluor 647], mAb, Mouse; His Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated His antibody; 647 conjugated His monoclonal antibody
Description The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.
THETM His Tag Antibody [iFluor 647], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Host Species Mouse
Conjugation iFluor 647
Immunogen A synthetic peptide HHHHHH coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity
THETM His Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal His-tagged proteins.
Conjugate Dye Characterization
Cat. No. Conjugate Ex (nm) Em (nm)
A01800 iFluor 488 491 514
A01801 iFluor 555 559 569
A01802 iFluor 647 654 674
Storage The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> His Tag Antibody [iFluor 647], MAb, Mouse
THE<sup>TM</sup> His Tag Antibody [iFluor 647], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or His-tagged protein transfected CHO cells (Red)
using THETM His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, 4 µg/ml).


Cat. No.
Product Name
Size
Price
Quantity
A00174-40 His-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00186-100 THETM His Tag Antibody, mAb, Mouse
100 ug
$375.00
A00205-40 His-tag antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00612 THETM His Tag Antibody [HRP], mAb, Mouse
100 ug
$410.00
A00613 THETM His Tag Antibody [Biotin], mAb, Mouse
100 ug
$410.00
A01620 THETM His Tag Antibody [FITC], mAb, Mouse
100 ug
$410.00
L00436 His Tag ELISA Detection Kit
1 kit
$298.00
L00439-1 Anti-His Affinity Resin
1 ml
$399.00
L00439-5 Anti-His Affinity Resin
5 ml
$1599.00
L00440c His Tag Antibody Plate (clear, 8x12 strips)
5 plates
$179.00
L00440w His Tag Antibody Plate (white, 96 wells, nonremovable)
5 plates
$179.00
L00440b His Tag Antibody Plate (black, 96 wells, nonremovable)
5 plates
$179.00
A01800-100 THETM His Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$450.00
A01801-100 THETM His Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$450.00
 


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Cat. No. A01802
Size
Price
100 ug $450.00
24 hour
 
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