THETM His Tag Antibody [iFluor 647], mAb, Mouse Antibody

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Overview

Synonyms	THE^TM His Tag Antibody [iFluor 647], mAb, Mouse; His Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated His antibody; 647 conjugated His monoclonal antibody
Description	The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins. THE^TM His Tag Antibody [iFluor 647], mAb, Mouse is THE^TM His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Host Species	Mouse
Conjugation	iFluor 647
Immunogen	A synthetic peptide HHHHHH coupled to KLH
Purification	Protein A affinity column

The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> His Tag Antibody [iFluor 647], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or His-tagged protein transfected CHO cells (Red)
using THE^TM His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, 4 µg/ml).

Related Products

Cat. No.	Product Name	Price
A00174-40	His-tag Antibody, pAb, Rabbit - 40 ug	$99.00
A00186-100	THE^TM His Tag Antibody, mAb, Mouse - 100 ug	$375.00
A00205-40	His-tag antibody [Biotin], pAb, Rabbit - 40 ug	$99.00
A00612	THE^TM His Tag Antibody [HRP], mAb, Mouse - 100 ug	$410.00
A00613	THE^TM His Tag Antibody [Biotin], mAb, Mouse - 100 ug	$410.00
A01620	THE^TM His Tag Antibody [FITC], mAb, Mouse - 100 ug	$410.00
L00436	His Tag ELISA Detection Kit - 1 kit	$298.00
L00439-1	Anti-His Affinity Resin - 1 ml	$399.00
L00439-5	Anti-His Affinity Resin - 5 ml	$1599.00
L00440c	His Tag Antibody Plate (clear, 8x12 strips) - 5 plates	$179.00
L00440w	His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates	$179.00
L00440b	His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates	$179.00
A01800-100	THE^TM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug	$450.00
A01801-100	THE^TM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug	$450.00