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THETM His Tag Antibody [iFluor 647], mAb, Mouse
THETM His Tag Antibody [iFluor 647], mAb, Mouse
| Synonyms | THETM His Tag Antibody [iFluor 647], mAb, Mouse; His Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated His antibody; 647 conjugated His monoclonal antibody |
| Description | The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins. THETM His Tag Antibody [iFluor 647], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647. |
| Host Species | Mouse |
| Conjugation | iFluor 647 |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Purification | Protein A affinity column |
| Subclass | IgG1, k | ||||||||||||||||
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide. | ||||||||||||||||
| Reconstitution | Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. | ||||||||||||||||
| Specificity |
THETM His Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal His-tagged proteins. Conjugate Dye Characterization
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| Storage | The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Immunofluorescent staining: 1-4 µg/ml Flow cytometry: 1-4 µg/ml Other applications: user-optimized |
![THE<sup>TM</sup> His Tag Antibody [iFluor 647], MAb, Mouse](/product/documents/down?doc_name=A01802 FACS.jpg&file=scm_files/productFile_notes/2013/12/16/623394e641c9a849b09d05fe6df0328c.jpg "THE<sup>TM</sup> His Tag Antibody [iFluor 647], MAb, Mouse")
Flow cytometric analysis of non-transfected CHO cells (Black) or His-tagged protein transfected CHO cells (Red)
using THETM His Tag Antibody [iFluor 647], mAb, Mouse (A01802-100, 4 µg/ml).
Cat. No. |
Product Name |
Price |
|
|---|---|---|---|
L00440w |
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates |
$89.5 $179.00 |
|
L00440c |
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates |
$89.5 $179.00 |
|
L00440b |
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates |
$89.5 $179.00 |
|
L00439-1 |
Anti-His Affinity Resin - 1 ml |
$399.00 |
|
L00439-5 |
Anti-His Affinity Resin - 5 ml |
$1599.00 |
|
L00436 |
His Tag ELISA Detection Kit - 1 kit |
$149 $298.00 |
|
A01801-100 |
THETM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug |
$450.00 |
|
A01800-100 |
THETM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug |
$450.00 |
|
A01620 |
THETM His Tag Antibody [FITC], mAb, Mouse - 100 ug |
$410.00 |
|
A00613 |
THETM His Tag Antibody [Biotin], mAb, Mouse - 100 ug |
$410.00 |
|
A00612 |
THETM His Tag Antibody [HRP], mAb, Mouse - 100 ug |
$410.00 |
|
A00205-40 |
His-tag antibody [Biotin], pAb, Rabbit - 40 ug |
$99.00 |
|
A00186-100 |
THETM His Tag Antibody, mAb, Mouse - 100 ug |
$375.00 |
|
A00174-40 |
His-tag Antibody, pAb, Rabbit - 40 ug |
$99.00 |
For more documents, please visit "Technical Support".
| Cat. No. A01802 | ||
|---|---|---|
Size |
Price |
|
| 100 ug | $450.00 | |
