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THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse

Name: THETM V5 Tag Antibody [iFluor 488], mAb, Mouse
SKU: A01803-100
Rating: 4.5 (10 reviews)

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THE<sup>TM</sup> V5 Tag Antibody [iFluor 488], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or V5-tagged protein transfected CHO cells (Red)
using THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse (A01803-100, 2 µg/ml).

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or V5-tagged protein transfected CHO cells (b)
using THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse (A01803-100, 4 µg/ml).

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Overview

Synonyms	THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse; V5 Tag Antibody [iFluor 488], mAb, Mouse; 488 conjugated V5 antibody; 488 conjugated V5 monoclonal antibody
Description	The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus, C-terminus or inserted in an internal region of the protein to facilitate detection and purification. An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins. THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse is THE^TM V5 Tag Antibody (A01724) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor488.
Host Species	Mouse
Conjugation	iFluor 488
Immunogen	A synthetic peptide GKPIPNPLLGLDST coupled to KLH
Purification	Protein A affinity column

Properties

Subclass

IgG2a, k

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.

Reconstitution

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

Specificity

THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse recognizes N-terminal, internal and C-terminal V5-tagged proteins.
Fluorescent Dye Characterization

Cat. No.	Conjugate	Ex (nm)	Em (nm)
A01803	iFluor 488	491	514
A01804	iFluor 555	559	569
A01805	iFluor 647	654	674

Storage

The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized

Examples

Flow cytometric analysis of non-transfected CHO cells (Black) or V5-tagged protein transfected CHO cells (Red)
using THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse (A01803-100, 2 µg/ml).

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