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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM V5 Tag Antibody [iFluor 555], mAb, Mouse

THETM V5 Tag Antibody [iFluor 555], mAb, Mouse

Synonyms THETM V5 Tag Antibody [iFluor 555], mAb, Mouse; V5 Tag Antibody [iFluor 555], mAb, Mouse; 555 conjugated V5 antibody; 555 conjugated V5 monoclonal antibody
Description The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus or inserted in an internal region of the protein to facilitate detection and purification. An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins.
THETM V5 Tag Antibody [iFluor 555], mAb, Mouse is THETM V5 Tag Antibody (A01724) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
Host Species Mouse
Conjugation iFluor 555
Immunogen A synthetic peptide GKPIPNPLLGLDST coupled to KLH
Purification Protein A affinity column

Subclass IgG2a, k
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM V5 Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal V5-tagged proteins.
Fluorescent Dye Characterization
Cat. No.
Conjugate
Ex (nm)
Em (nm)

A01803

iFluor 488

491

514

A01804

iFluor 555

559

569

A01805

iFluor 647

654

674

Storage The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Other applications: user-optimized

  • THE<sup>TM</sup> V5 Tag Antibody [iFluor 555], MAb, Mouse
  • THE<sup>TM</sup> V5 Tag Antibody [iFluor 555], MAb, Mouse

    Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) and V5-tagged protein transfected CHO cells (b)
    using THETM V5 Tag Antibody [iFluor 555], mAb, Mouse (A01804-100, 4 µg/ml).


Cat. No.
Product Name
Price
A01805-100
THETM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$263.00
A01803-100
THETM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$263.00
A01735-100
THETM V5 Tag Antibody [Biotin], mAb, Mouse - 100 ug
$239.00
A01733-100
THETM V5 Tag Antibody [HRP], mAb, Mouse - 100 ug
$239.00
A01724-40
THETM V5 Tag Antibody, mAb, Mouse - 40 ug
$119.00
A01724-100
THETM V5 Tag Antibody, mAb, Mouse - 100 ug
$219.00
A01724-200
THETM V5 Tag Antibody, mAb, Mouse - 200 ug
$395.00
A00877-40
V5-tag Antibody [HRP], pAb, Rabbit - 40 ug
$99.00
A00877-100
V5-tag Antibody [HRP], pAb, Rabbit - 100 ug
$199.00
A00623-40
V5-tag Antibody, pAb, Rabbit - 40 ug
$99.00
 


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Cat. No. A01804
Size
Price
100 ug $263.00