THETM V5 Tag Antibody [iFluor 555], mAb, Mouse Antibody

Collapse all
Expand all

Overview

Synonyms	THE^TM V5 Tag Antibody [iFluor 555], mAb, Mouse; V5 Tag Antibody [iFluor 555], mAb, Mouse; 555 conjugated V5 antibody; 555 conjugated V5 monoclonal antibody
Description	The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus or inserted in an internal region of the protein to facilitate detection and purification. An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins. THE^TM V5 Tag Antibody [iFluor 555], mAb, Mouse is THE^TM V5 Tag Antibody (A01724) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
Host Species	Mouse
Conjugation	iFluor 555
Immunogen	A synthetic peptide GKPIPNPLLGLDST coupled to KLH
Purification	Protein A affinity column

The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> V5 Tag Antibody [iFluor 555], MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) and V5-tagged protein transfected CHO cells (b)
using THE^TM V5 Tag Antibody [iFluor 555], mAb, Mouse (A01804-100, 4 µg/ml).

Related Products

Cat. No.	Product Name	Price
A00877-40	V5-tag Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
A01724-40	THE^TM V5 Tag Antibody, mAb, Mouse - 40 ug	$119.00
A01724-100	THE^TM V5 Tag Antibody, mAb, Mouse - 100 ug	$219.00
A01724-200	THE^TM V5 Tag Antibody, mAb, Mouse - 200 ug	$395.00
A01733-100	THE^TM V5 Tag Antibody [HRP], mAb, Mouse - 100 ug	$239.00
A01735-100	THE^TM V5 Tag Antibody [Biotin], mAb, Mouse - 100 ug	$239.00
A00877-100	V5-tag Antibody [HRP], pAb, Rabbit - 100 ug	$199.00
A01803-100	THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug	$263.00
A01805-100	THE^TM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug	$263.00