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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM HA Tag Antibody [iFluor 647], mAb, Mouse

THETM HA Tag Antibody [iFluor 647], mAb, Mouse

Synonyms THETM HA Tag Antibody [iFluor 647], mAb, Mouse; HA Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated HA antibody; 647 conjugated HA monoclonal antibody
Description The HA tag is a polypeptide with the amino acid sequence YPYDVPDYA that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-HA tag antibody is a useful tool for the analysis of HA-tagged proteins.
THETM HA Tag Antibody [iFluor 647], mAb, Mouse is THETM HA Tag Antibody (A01244) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of HA-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Host Species Mouse
Conjugation iFluor 647
Immunogen A synthetic peptide YPYDVPDYA coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM HA Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal HA-tagged proteins.
Fluorescent Dye Characterization
Cat. No.
Conjugate
Ex (nm)
Em (nm)

A01806

iFluor 488

491

514

A01807

iFluor 555

559

569

A01808

iFluor 647

654

674

Storage The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> HA Tag Antibody [iFluor 647], MAb, Mouse
THE<sup>TM</sup> HA Tag Antibody [iFluor 647], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or HA-tagged protein transfected CHO cells (Red)
using THETM HA Tag Antibody [iFluor 647], mAb, Mouse (A01808-100, 4 µg/ml).


Cat. No.
Product Name
Size
Price
Quantity
A00168-40 HA-tag Antibody, pAb, Goat
40 ug
$99.00
A00169-100 HA-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
A00169-40 HA-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
A00203-40 HA-tag Antibody [Biotin], pAb, Goat
40 ug
$99.00
A01244-100 THETM HA Tag Antibody, mAb, Mouse
100 ug
$287.00
A01296 THETM HA Tag Antibody [HRP], mAb, Mouse
100 ug
$299.00
A01297 THETM HA Tag Antibody [Biotin], mAb, Mouse
100 ug
$299.00
A01621 THETM HA Tag Antibody [FITC], mAb, Mouse
100 ug
$299.00
A01806-100 THETM HA Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$344.00
A01807-100 THETM HA Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$344.00
 


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Cat. No. A01808
Size
Price
100 ug $344.00
24 hour
 
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