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Catalog Products » Antibodies » Epitope Tag Antibodies » THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse

THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse

THE™ DYKDDDDK Tag Antibody [iFluor 555], MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
using THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse (A01810-100, 4 µg/ml).

Specificity THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.
Host Species Mouse
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Conjugate iFluor 555

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
Immunofluorescent staining 1-4 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A affinity column
Isotype Mouse IgG2b,κ
Clonality Monoclonal
Clone ID 5A8E5

  • THE™ DYKDDDDK Tag Antibody [iFluor 555], MAb, Mouse
  • THE™ DYKDDDDK Tag Antibody [iFluor 555], MAb, Mouse

    Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
    using THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse (A01810-100, 4 µg/ml).


Target Background The Flag tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
Synonyms THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; 555 conjugated Flag antibody; 555 conjugated Flag monoclonal antibody;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Cat. No. Product Name Quantity Price
L00455W
DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates
$229.00
L00455C
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates
$229.00
L00455B
DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates
$229.00
L00432-1
Anti-DYKDDDDK G1 Affinity Resin - 1 ml Resin (Total 2 ml)
$281.00
L00432-25
Anti-DYKDDDDK G1 Affinity Resin - 25 ml Resin (Total 50 ml)
$2591.00
L00432-5
Anti-DYKDDDDK G1 Affinity Resin - 5 ml Resin (Total 10 ml)
$663.00
L00432-10
Anti-DYKDDDDK G1 Affinity Resin - 10 ml Resin (Total 20 ml)
$1193.00
L00425
Anti-DYKDDDDK IP Resin - 1 ml Resin (Total 2 ml)
$199.00
A01811-100
THE™ DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse - 100 μg
$167.00
A01809-100
THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
$167.00
A01632
THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100 μg
$149.00
A01429-100
THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 μg
$149.00
A01428-100
THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 μg
$149.00
A00187-100
THE™ DYKDDDDK Tag Antibody, mAb, Mouse - 100 μg
$139.00
A00187-200
THE™ DYKDDDDK Tag Antibody, mAb, Mouse - 200 μg
$256.00
A00170-40
DYKDDDDK-tag Antibody, pAb, Rabbit - 40 μg
$99.00
 


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Cat. No. A01810
Size Price
100 μg $167.00