Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
using THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse (A01810-100, 4 µg/ml).
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| Specificity | THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide DYKDDDDK coupled to KLH |
| Conjugate | iFluor 555 |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||
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| Form | Lyophilized |
| Storage Buffer | lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions | The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2b,κ |
| Clonality | Monoclonal |
| Clone ID | 5A8E5 |
Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
using THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse (A01810-100, 4 µg/ml).
| Target Background | The Flag tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555. |
| Synonyms | THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; 555 conjugated Flag antibody; 555 conjugated Flag monoclonal antibody; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
