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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse

THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse

Synonyms THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse; 555 conjugated Flag antibody; 555 conjugated Flag monoclonal antibody;
Description The Flag tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.
THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
Host Species Mouse
Conjugation iFluor 555
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Purification Protein A affinity column

Subclass IgG2b, k
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.
Fluorescent Dye Characterization
Cat. No.
Conjugate
Ex (nm)
Em (nm)

A01809

iFluor 488

491

514

A01810

iFluor 555

559

569

A01811

iFluor 647

654

674

Storage The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

THE<sup>TM</sup> DYKDDDDK Tag Antibody [iFluor 555], MAb, Mouse
THE<sup>TM</sup> DYKDDDDK Tag Antibody [iFluor 555], MAb, Mouse

Immunocytochemistry/Immunofluorescence analysis of non-transfected CHO cells (a) or Flag-tagged protein transfected CHO cells (b)
using THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse (A01810-100, 4 µg/ml).


Cat. No.
Product Name
Size
Price
Quantity
A00170-40 DYKDDDDK-tag Antibody, pAb, Rabbit
40 ug
$99.00
A00187-100 THETM DYKDDDDK Tag Antibody, mAb, Mouse
100 ug
$139.00
A00187-200 THETM DYKDDDDK Tag Antibody, mAb, Mouse
200 ug
$256.00
A01428-100 THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse
100 ug
$149.00
A01429-100 THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse
100 ug
$149.00
A01632 THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse
100 ug
$149.00
L00425 Anti-DYKDDDDK IP Resin
1 ml
$199.00
L00432-1 Anti-DYKDDDDK G1 Affinity Resin
1 ml
$281.00
L00432-25 Anti-DYKDDDDK G1 Affinity Resin
25 ml
$2591.00
L00432-5 Anti-DYKDDDDK G1 Affinity Resin
5 ml
$663.00
L00432-10 Anti-DYKDDDDK G1 Affinity Resin
10 ml
$1193.00
L00455C DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip)
5 plates
$229.00
L00455W DYKDDDDK Tag Antibody Plate (White, 96-well)
5 plates
$229.00
L00455B DYKDDDDK Tag Antibody Plate (Black, 96-well)
5 plates
$229.00
A01809-100 THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$167.00
A01811-100 THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$167.00
 


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Cat. No. A01810
Size
Price
100 ug $167.00
24 hour
 
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