THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse Antibody

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Overview

Synonyms	THE^TM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated Flag antibody; 647 conjugated Flag monoclonal antibody;
Description	The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins. THE^TM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse is THE^TM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Host Species	Mouse
Conjugation	iFluor 647
Immunogen	A synthetic peptide DYKDDDDK coupled to KLH
Purification	Protein A affinity column

The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Examples

THE<sup>TM</sup> DYKDDDDK Tag Antibody [iFluor 647], MAb, Mouse

Flow cytometric analysis of non-transfected CHO cells (Black) or Flag-tagged protein transfected CHO cells (Red)
using THE^TM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse (A01811-100, 4 µg/ml).

Related Products

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L00455C	DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates	$229.00
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