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Catalog Products » Antibodies » Epitope Tag Antibodies » THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse

THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse

Synonyms THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse; DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated Flag antibody; 647 conjugated Flag monoclonal antibody;
Description The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.
THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
Host Species Mouse
Conjugation iFluor 647
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Purification Protein A affinity column

Subclass IgG2b, k
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal DYKDDDDK-tagged proteins.
Fluorescent Dye Characterization
Cat. No.
Conjugate
Ex (nm)
Em (nm)

A01809

iFluor 488

491

514

A01810

iFluor 555

559

569

A01811

iFluor 647

654

674

Storage The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized

  • THE<sup>TM</sup> DYKDDDDK Tag Antibody [iFluor 647], MAb, Mouse
  • THE<sup>TM</sup> DYKDDDDK Tag Antibody [iFluor 647], MAb, Mouse

    Flow cytometric analysis of non-transfected CHO cells (Black) or Flag-tagged protein transfected CHO cells (Red)
    using THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse (A01811-100, 4 µg/ml).


Cat. No.
Product Name
Price
L00455W
DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates
$229.00
L00455C
DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates
$229.00
L00455B
DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates
$229.00
L00432-1
Anti-DYKDDDDK G1 Affinity Resin - 1 ml
$281.00
L00432-25
Anti-DYKDDDDK G1 Affinity Resin - 25 ml
$2591.00
L00432-5
Anti-DYKDDDDK G1 Affinity Resin - 5 ml
$663.00
L00432-10
Anti-DYKDDDDK G1 Affinity Resin - 10 ml
$1193.00
L00425
Anti-DYKDDDDK IP Resin - 1 ml
$199.00
A01810-100
THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$167.00
A01809-100
THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$167.00
A01632
THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100 ug
$149.00
A01429-100
THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 ug
$149.00
A01428-100
THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 ug
$149.00
A00187-100
THETM DYKDDDDK Tag Antibody, mAb, Mouse - 100 ug
$139.00
A00187-200
THETM DYKDDDDK Tag Antibody, mAb, Mouse - 200 ug
$256.00
A00170-40
DYKDDDDK-tag Antibody, pAb, Rabbit - 40 ug
$99.00
 


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Cat. No. A01811
Size
Price
100 ug $167.00