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Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » Phosphotyrosine Antibody (5E10), mAb, Mouse

Phosphotyrosine Antibody (5E10), mAb, Mouse

Synonyms Mouse monoclonal to Phosphotyrosine/pY
Description Phosphotyrosine is a tyrosine residue covalently bound to a phosphate via its hydroxyl group. A tyrosine kinase catalyzes the transfer of a phosphate from ATP to a tyrosine residue on a protein substrate. Tyrosine phosphorylation plays a key role in intracellular signaling and cancer development. Many tyrosine kinases are drug targets for different forms of cancer. Phosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuable tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery.
GenScript Phosphotyrosine Antibody (5E10), mAb, Mouse is produced from a hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with phosphotyrosine conjugated to KLH.
Host Species Mouse
Conjugation Unconjugated
Immunogen Phosphotyrosine containing carrier protein
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 5E10E1
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity The antibody is specific for proteins containing phosphotyrosine residues. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues.
Storage The lyophilized product remains stable up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western Blot: 1 µg/ml.
Other applications: user-optimized

  • Phosphotyrosine Antibody (5E10), MAb, Mouse
  • Phosphotyrosine Antibody (5E10), MAb, Mouse

    Western blot analysis of EGF-stimulated A431 cell lysates and untreated A431 cell lysates with Phosphotyrosine Antibody (5E10), mAb, Mouse (GenScript, A01817, 1 µg/ml).
    The signal was developed with IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

Cat. No.
Product Name
THETM Phosphotyrosine Antibody (E10)plus - 100 ug
THETM ADP Antibody, mAb, Mouse - 40 ug
THETM ADP Antibody, mAb, Mouse - 100 ug
ADP Antibody, pAb, Rabbit - 40 μl
ADP Antibody, pAb, Rabbit - 200 μl

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Cat. No. A01817
100 ug $199.00