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Catalog Products » Antibodies » MonoRab™ Rabbit Monoclonal Antibody Product » MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), mAb, Rabbit

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Sensitivity and specificity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) to 6xHis-tag Fusion Protein by Western blot.

Loading: His tagged fusion protein
Primary Antibody:
MonoRabTM Anti-His Tag Antibody (A01857-40) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)

Loading:
Lane 1: MonoRabTM Anti-His antibody (4 μg) + His tagged fusion protein
Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
Lane 3: His tagged fusion protein (Input)
Primary Antibody:
THETM His tag antibody, mAb, Mouse (Genscript,A01857) 0.2 μg/ml
Secondary Antibody:
Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

Western blot analysis of cross-reactivity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)
Primary Antibody:
A: MonoRabTM Anti-His Tag Antibody (A01857-40) 1μg/ml
B: Rabbit IgG (Negative control) 1μg/ml
Secondary Antibody:
Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211)0.125μg/ml

Specificity GenScript MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit specifically recognizes His tags placed at C-terminal and internal regions of His fusion proteins. This product is not recommended for N-terminal His fusion protein detection.
Host Species Rabbit
Immunogen 6x His synthetic peptide coupled to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 μg/ml
Western Blot 0.1-0.2 μg/ml 
Immunoprecipitation (IP) 2 μg/ml 
Immunofluorescent staining 1 μg/ml 
Flow Cytometry (FACS) 1 μg/ml 
Other applications User-optimized

Form Lyophilized
Storage Buffer lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL.
Storage Instructions The lyophilized product remains stable up to 1 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freeze and thaw cycles.
Purification Protein A affinity column
Isotype Rabbit IgG
Clonality Monoclonal
Clone ID 25B6E11
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

    Sensitivity and specificity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40) to 6xHis-tag Fusion Protein by Western blot.

    Loading: His tagged fusion protein
    Primary Antibody:
    MonoRabTM Anti-His Tag Antibody (A01857-40) 0.2 μg/ml
    Secondary Antibody:
    Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

    Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)

    Loading:
    Lane 1: MonoRabTM Anti-His antibody (4 μg) + His tagged fusion protein
    Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
    Lane 3: His tagged fusion protein (Input)
    Primary Antibody:
    THETM His tag antibody, mAb, Mouse (Genscript,A01857) 0.2 μg/ml
    Secondary Antibody:
    Goat anti-rabbit IgG (H&L) [IRDye800] (Licor,926-32211) 0.125 μg/ml

  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit
  • MonoRab™ Anti-His Tag (C-term) Antibody(25B6E11), MAb, Rabbit

    Western blot analysis of cross-reactivity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit (A01857-40)
    Primary Antibody:
    A: MonoRabTM Anti-His Tag Antibody (A01857-40) 1μg/ml
    B: Rabbit IgG (Negative control) 1μg/ml
    Secondary Antibody:
    Goat anti-Rabbit IgG (H&L) [IRDye800] (Licor,926-32211)0.125μg/ml


Target Background The rabbit immune system generates antibody diversity and optimizes affinity. GenScript utilizes MonoRabTM technology to generate high affinity and specific monoclonal rabbit antibodies. GenScript MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Synonyms Rabbit monoclonal to His tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.






For more documents, please visit "Technical Support".

Cat. No. A01857
Size Price
40 μg $198.00